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599 p3E-mcs1

Plasmid

#49004

Purpose

Multiple cloning site in 3' gateway entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Type

Empty backbone

Use

Gateway 3' entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Publication

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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p5E--3mnx1

Plasmid

#74632

Purpose

Can be used to drive expression specifically in zebrafish motor neurons

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

-3mnx1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ACE2

Plasmid

#164219

Purpose

Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

Depositor

Sriram Neelamegham

Publication

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Mutation

silent mutation to remove internal EcoRI site (GA…

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Publication

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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PpyCAG-KLF2-IB

Plasmid

#60441

Purpose

Transient human KLF2 expression

Depositor

Austin Smith

Publication

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KLF2 (KLF2 Human)

Expression

Mammalian

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDule-pCNF

Plasmid

#85494

Purpose

Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

Depositor

Ryan Mehl

Publication

Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

Insert

para-cyanophenylalanine Mj synthetase

Tags

None

Expression

Bacterial

Mutation

Y32L L65V F108W Q109M D158G I159P

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MAC-NRP1

Plasmid

#158384

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

NRP1 (NRP1 Human)

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pRSET-4xTR-TUBE

Plasmid

#110312

Purpose

E. coli expression and purification of 4xTR-TUBE

Depositor

Yasushi Saeki

Publication

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

4xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLEW13

Plasmid

#24007

Depositor

George Cross

Insert

T7 RNA Polymerase

Use

Trypanosome expression

Available since

May 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p5E--3mnx1

Plasmid

#74632

Purpose

Can be used to drive expression specifically in zebrafish motor neurons

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

-3mnx1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ACE2

Plasmid

#164219

Purpose

Expresses full length human ACE2 protein, along with TagBFP reporter and Puromycin selection marker

Depositor

Sriram Neelamegham

Publication

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Mutation

silent mutation to remove internal EcoRI site (GA…

Promoter

CMV

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Publication

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Publication

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEVOL-AckRS

Plasmid

#137976

Purpose

sequence optimized N‐acetyl lysyl‐tRNA synthetase with cognate tRNA for genetic code expansion

Depositor

Wenshe Liu

Publication

Wang et al J Am Chem Soc. 2019 Feb 13;141(6):2462-2

Insert

AckRS and pylTcua

Expression

Bacterial

Promoter

araBAD

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Trip-GFP-hOCLN

Plasmid

#32789

Depositor

Tianyi Wang

Publication

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLiLucB

Plasmid

#31596

Depositor

Michele Calos

Publication

Woodard et al PLoS One. 2010 . 5(6):e11367.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)

Use

Luciferase

Expression

Mammalian

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Publication

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pHAGE-EF1alphaL-hAAT-W

Plasmid

#24527

Purpose

Lentiviral overexpression

Depositor

Darrell Kotton

Publication

Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

Insert

alpha-1 antitrypsin (SERPINA1 Human)

Use

Lentiviral

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NowGFP /pQE-30

Plasmid

#74749

Purpose

NowGFP gene for bacterial expression

Depositor

Konstantin Lukyanov

Publication

Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

Insert

NowGFP

Tags

HisTag (with a mutation but still working)

Expression

Bacterial

Mutation

WasCFP with substitutions E6K, L42M, T43S, V68M, …

Promoter

T5

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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