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pmCherry-U6-CLIC3

Plasmid

#140583

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA CLIC3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-SYS1-msGFPx3

Plasmid

#177112

Purpose

Expresses Sys1-msGFP3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SYS1 (SYS1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Rapr-FAK

Plasmid

#25926

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only
pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

Plasmid

#203911

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C2]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

Plasmid

#203910

Purpose

Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

Depositor

Jared Toettcher

Article

Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

Insert

ZF9LOV[C1]-VP64

Expression

Mammalian

Promoter

EF1α

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-puro

Plasmid

#208031

Purpose

Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

Plasmid

#50031

Purpose

Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

PM-SrtA(strep)

Tags

C-HA

Expression

Mammalian

Promoter

pCMV

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx1

Plasmid

#158227

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 (Prx1) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx1

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-EMX1

Plasmid

#140581

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA EMX1

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLPCX mCER-Prx2

Plasmid

#158225

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for stable cell line generation)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Use

Retroviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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MSGV Rh Acceptor

Plasmid

#64271

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus Macaque T-Cell Receptor genes

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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p3XFlag-YedK

Plasmid

#84567

Purpose

Expresses YedK in mammalian cells

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK

Tags

3xFlag and C-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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zCREST3:Cas9green; T3_gRNA

Plasmid

#199335

Purpose

pDEL161; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-EMX1+38

Plasmid

#140579

Purpose

Expresses a pegRNA in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

pegRNA EMX1 +38

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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mGarnet-RITA

Plasmid

#104313

Purpose

codes for a fusion of fluorescent protein mGarnet and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cells

Depositor

Ulrich Nienhaus

Article

Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

Insert

mGarnet-RITA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Scarlet-I

Plasmid

#193554

Purpose

Expresses mEGFP-mScarlet-I in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mScarlet-I

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Elk1 pShuttle

Plasmid

#121097

Purpose

Expression of full length ELK1 in mammalian cells

Depositor

Paul Herring

Article

Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

Insert

ELK1 (Elk1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-msGFP2-STE13

Plasmid

#177106

Purpose

Expresses msGFP2-Ste13 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

STE13 (STE13 Budding Yeast)

Expression

Yeast

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-A114-N40-HhR

Plasmid

#50555

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-A114-N40-HhR

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-VEGFA

Plasmid

#140588

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA VEGFA

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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