We narrowed to 18,088 results for: Jun
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Plasmid#153432PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2DepositorInsertCTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSYFP2Available SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-223-3p
Plasmid#103369PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-223-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-223-3p target (MIR223 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4t1 RARalpha (1-187)
Plasmid#35558DepositorAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tyk2-SH2
Plasmid#46527DepositorInsertTyk2 (TYK2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 452-567PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRE Tight ArchT-GFP
Plasmid#79627PurposeTetracycline-dependent expression of ArchT-EGFP in mammalian cellsDepositorInsertArchT
TagsEGFPExpressionMammalianMutationArchT is a more sensitive version of Arch (archae…PromoterTet responsive Ptight promoterAvailable SinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_TSS1
Plasmid#210633PurposeLuciferase experiment for TAL1 TSS1DepositorInsertTAL1 TSS1 (TAL1 Human)
ExpressionMammalianAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_TSS2
Plasmid#210634PurposeLuciferase experiment for TAL1 TSS2DepositorInsertTAL1 TSS2 (TAL1 Human)
ExpressionMammalianAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_TSS4
Plasmid#210635PurposeLuciferase experiment for TAL1 TSS4DepositorInsertTAL1 TSS4 (TAL1 Human)
ExpressionMammalianAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23_TSS5
Plasmid#210636PurposeLuciferase experiment for TAL1 TSS5DepositorInsertTAL1 TSS5 (TAL1 Human)
ExpressionMammalianAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
J23117-mRFP1-331Bb
Plasmid#78280PurposemRFP1 behind constitutive promoter J23117 in pSEVA331Bb backboneDepositorInsertJ23117-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB65 - pL0_tGFP-I (CDS1)
Plasmid#123182PurposeGolden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)DepositorInsertturboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
UsePart for plant expressionExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7a-2-3p
Plasmid#103144PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7a-2-3p target (MIRLET7A2 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nck-SH2
Plasmid#46457DepositorInsertNck (NCK1 Human)
TagsGST and Thrombin siteExpressionBacterialMutationcontains amino acids 275-377PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX 4T1 RARalpha (186-462)
Plasmid#35559DepositorAvailable SinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-148a-3p
Plasmid#103250PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-148a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-148a-3p target (MIR148A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-BioID2-HA
Plasmid#130980PurposeExpression of HA-tagged KIF1C-BioID2DepositorInsertKIF1C-BioID2-HA (KIF1C Human)
TagsBioID2-HAExpressionMammalianMutation5 silent mutations that make this construct RNAi …PromoterCMVAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G
Plasmid#157984PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G
Plasmid#157985PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-155-3p
Plasmid#103264PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-155-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-155-3p target (MIR155 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
His6tev CBP BCHZDeltaAL
Plasmid#99551PurposeBacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSGDepositorInsertmouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)
TagsHis6tevExpressionBacterialMutationreplaced 1557-1618 with SGGSGPromoterT7Available SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only