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pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Expression

Bacterial

Mutation

R632S

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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EF1m-ELP

Plasmid

#170763

Purpose

EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

Depositor

Andrew MacKay

Article

Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

Insert

EF1m-ELP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pGL0_7 [pSa ORI]

Plasmid

#198923

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSa ORI

Use

Synthetic Biology

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBbE2k-pTet-gRNAwcaF159

Plasmid

#122259

Purpose

aTc inducible gRNA expression plasmid

Depositor

Chueh Loo Poh

Article

Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

Insert

gRNAwcaF159

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-GYB

Plasmid

#160927

Purpose

Dual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Inserts

BFP
sfGFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_NXF1_TAP-C

Plasmid

#110038

Purpose

Protein expression and purification of NXF1_TAP-C

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NXF1_TAP-C (NXF1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR6A1_Hormone-recep

Plasmid

#109945

Purpose

Protein expression and purification of NR6A1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR6A1_Hormone-recep

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-TFE3pA

Plasmid

#120201

Purpose

Human TFE3 poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-ccdb-mKate2

Plasmid

#109731

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-actc1b-mKate2-ccdb

Plasmid

#109732

Purpose

Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Type

Empty backbone

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-ATG18

Plasmid

#109556

Purpose

Multisite gateway vector for 3' tagging with yeast ATG18 (PLEKHA3). Binds PtdIns(3,5P)2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

yeast ATG18 (PLEKHA3)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G(P)7G

Plasmid

#137100

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(P)7G

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFD-FKBP12

Plasmid

#137108

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

FKBP12

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G3

Plasmid

#137092

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GGG

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXO15_WT

Plasmid

#81839

Purpose

Gateway Donor vector containing FBXO15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXO15 (FBXO15 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM11_WT

Plasmid

#81784

Purpose

Gateway Donor vector containing RBM11 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM11 (RBM11 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - SodB

Plasmid

#89613

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase B

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBN79

Plasmid

#86717

Purpose

DamID plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::Dam::MYC::Emr-1

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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