We narrowed to 31,821 results for: LIS;

1. human SLC12A5-V5-HA-2A-Luciferase repair template

   Plasmid

   #158576

   Purpose

   For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

   Depositor

   Rudolf Jaenisch

   Article

   Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

   Insert

   human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
   

   Tags

   V5 and HA on the endogenous KCC2 protein product

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV phSyn1(S)-FlpE-bGHpA

   Plasmid

   #50364

   Purpose

   Can be used to generate AAV virus that will express FlpE recombinse gene in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   FlpE recombinase gene
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TP53-KO_gRNA_2

   Plasmid

   #195131

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 2 (TP53 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. HH06-LP Clone 6 heavy chain

   Plasmid

   #192176

   Purpose

   Clone 6 heavy chain (HH06)

   Depositor

   Sidi Chen

   Article

   Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

   Insert

   Clone 6 heavy chain (HH06)
   

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCCL-PGK-SPdCas9-BFP-EGFP

   Plasmid

   #66821

   Purpose

   dCas9 fused to BFP and EGFP

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-BFP-EGFP
   

   Tags

   dSpCas9-BFP-EGFP

   Expression

   Mammalian

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-EglN2 H358A-pBabe-puro

   Plasmid

   #22705

   Depositor

   William Kaelin

   Article

   Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

   Insert

   Egg laying Nine - 2 (EglN2) (EGLN2 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   mutated cDNA: point mutation in codon 358 changi…

   Available Since

   Dec. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY164

   Plasmid

   #184140

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R3, PrhaB, and Ptet

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX028

   Plasmid

   #167151

   Purpose

   MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   p35s-nnHispS-ocsT
   

   Expression

   Plant

   Promoter

   p35s

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCardinal-Fibrillarin-7

   Plasmid

   #56160

   Purpose

   Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   March 4, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCBh_3x Flag-NLS-RhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid

    #197025

    Purpose

    Vector encoding a human codon-optimized RhCas12f1 driven by CBh promoter, guide RNAs compatible with RhCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized RhCas12f1
    

    Expression

    Mammalian

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgCdkn2a#1/Cre

    Plasmid

    #173629

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-sgCdkn2a#2/Cre

    Plasmid

    #173630

    Purpose

    Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Cdkn2a (Cdkn2a Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-sgNcor1#2/Cre

    Plasmid

    #173606

    Purpose

    Expresses a Ncor1-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Ncor1 (Ncor1 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-KIF5A delta Tail

    Plasmid

    #166955

    Purpose

    Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    KIF5A (1-820aa)

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97310

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, EF1a

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT-HGP-v3-Nb127D01-hIgG

    Plasmid

    #171564

    Purpose

    Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
    

    Tags

    BiP signal peptide and hIgG1-hinge-N297A-TEV site…

    Expression

    Insect

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-DEUT RUVBL1 Delete

    Plasmid

    #159144

    Purpose

    Expression and protien purification of mutated RUVBL1

    Depositor

    Eric Erquan Zhang

    Article

    Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

    Insert

    RUVBL1 (RUVBL1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acid 1-463, Glu134-Glu237 mutated to GPPG

    Available Since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-CMV-XRCC1-gRNA res-Neo

    Plasmid

    #176149

    Purpose

    XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    X-ray repair cross complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

    Promoter

    CMV

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-707pUC19-tNGFR-P2A-TTC37

    Plasmid

    #186122

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    TTC37 (SKIC3 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX018

    Plasmid

    #167144

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    fungal luciferase, nnLuz
    

    Use

    Luciferase and Synthetic Biology

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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