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pRCA595 - pBA904 Puro-T2A-mCherry

Plasmid

#238171

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mCherry

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-W

Plasmid

#238064

Purpose

Expresses rat CENP-W; untagged; made for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Rat CENP-W (Cenpw Rat)

Expression

Mammalian

Promoter

T7

Available Since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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BtA3N

Plasmid

#238981

Purpose

In vitro transcription of bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

arrestin-3 short (ARR3 Bovine)

Use

In vitro transcription

Mutation

silent, Cla I side introduced before stop codon

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pOP823

Plasmid

#235718

Purpose

Protein expression of His6-SUMO-HsRAB5A (Q79L C19S,C63S) 1-212 in bacteria for maleimide conjugation

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

RAB5A (RAB5A Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Q79L, C19S, C63S, aa 1-212

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pICH47811_AvrSr62-7

Plasmid

#233534

Purpose

Expresses AvrSr62-7 in protoplasts

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

AvrSr62-7

Use

Protoplast expression

Tags

Luciferase

Expression

Plant

Promoter

Ubiquitin

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMIIS794

Plasmid

#238207

Purpose

Expresses native TaTasR TIGR locus, TasR has premature stop codon. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Mutation

A insertion at K85 codon

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-GFP-hCGNL1

Plasmid

#236521

Purpose

For expression of Paracingulin (human) in insect cells

Depositor

Sandra Citi

Article

Flinois et al MicroPubl Biol. 2024 Oct 14;2024:10.1791

Insert

CGNL1 (CGNL1 Human)

Tags

2xStrep-10xHis-Tev-EGFP

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RFP-SIKE

Plasmid

#235691

Purpose

Express RFP tagged SIKE gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SIKE (SIKE1 Human)

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAPH07

Plasmid

#234345

Purpose

Library-scale IPTG-inducible gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

Depositor

Jerome Fox

Article

Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

Insert

Type II dCas9 sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAPH021

Plasmid

#234346

Purpose

Library-scale IPTG-inducible single transcript dual-gRNA expression for Type II dCas9 in Synechococcus sp. PCC 7002, genomically integrated next to glpK

Depositor

Jerome Fox

Article

Hren et al Proc Natl Acad Sci U S A. 2025 Mar 25;12

Inserts

Type II dCas9 sgRNA
Type II dCas9 sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

lacUV5

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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p3A3-Flag

Plasmid

#234805

Purpose

Mammalian expression of bovine arrestin-3 with C-terminal Flag tag

Depositor

Vsevolod Gurevich

Article

Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Flag

Expression

Mammalian

Mutation

none

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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psiCHECK2_Rluc-Y0x-Fluc

Plasmid

#233614

Purpose

Expression of Rluc-Fluc reporter gene containing no Y

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Rluc-Y0x-Fluc

Use

Luciferase

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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HA-cNM2C

Plasmid

#236535

Purpose

For expression of NM2C (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH14

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HA-cNM2B-Flag

Plasmid

#236534

Purpose

For expression of NM2B (dog) - with HA and FLAG tags in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

MYH10

Tags

FLAG and HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1645

Plasmid

#235719

Purpose

Protein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteria

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

p40phox PX domain (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

Cys at 2, C85S, aa 2-149

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP

Plasmid

#236134

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW408 (ZNF35tar-compact)x8-H2B-GFP

Plasmid

#236129

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight ZNF35 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight ZNF35 binding sit…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW369 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)

Plasmid

#236145

Purpose

Plasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sites

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW407 (ZNF35/ZNF35-6bp spacers)x4-H2B-GFP

Plasmid

#236137

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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