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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,964 results for: nin

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Showing: 8721 - 8740 of 66964 results

  2. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  3. pDEST-CMV 3xFLAG-LC3C-GFP

    Plasmid
    #123110
    Purpose
    Expresses 3xFLAG-LC3C-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3C
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123110-plasmid-map-sequence-id-244373

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  4. PMXS-GOT1

    Plasmid
    #72872
    Purpose
    The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.
    Depositor
    David Sabatini
    Publication
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    GOT1 (GOT1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    sgRNA resistant human GOT1, 7 silent mutations c1…
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. PEA1-Nuclease-Puro

    Plasmid
    #171992
    Purpose
    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171992-plasmid-map-sequence-id-338139

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  11. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140000-plasmid-map-sequence-id-275479

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  14. pTol2p2A--3mnx1:loxP-mTagBFP2-stop-loxP-mCherry-NS-MCS

    Plasmid
    #78251
    Purpose
    Can be used to subclone in tagged gene of interest into inducible motor neuron expression vector.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    -3mnx1 (mnx1 Zebrafish)
    -loxP-mTagBFP2-stop-
    mCherry-NS-MCS
    Use
    Zebrafish motor neuron inducible expression
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    78251-plasmid-map-sequence-id-149769

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  15. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid
    #55687
    Purpose
    expresses constitutively active human PKCi tagged with V5
    Depositor
    Pedro Salas
    Publication
    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    A120E
    Promoter
    CMV
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  20. AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)

    Plasmid
    #197509
    Purpose
    Cbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    ABE8e mutations in TadA and nSpCas9(D10A)
    Promoter
    Cbh
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197509-plasmid-map-sequence-id-391242

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Showing: 8721 - 8740 of 66964 results