-
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
UseTagsHA and tagBFPExpressionMammalianMutationPromoterSV40Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (mCherry)
Plasmid#102945PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (GFP)
Plasmid#102944PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBD-IFP2.0C
Plasmid#215748PurposeExpresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensorsDepositorInsertIFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBD_R44Q-IFP2.0C
Plasmid#215749PurposeExpresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensorsDepositorInsertIFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD-IFP2.0C
Plasmid#215750PurposeExpresses double domain of HP1beta chromo domain fused to C-terminal part of IFP2.0 for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag(22aa)
Plasmid#215745PurposeExpresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkersDepositorInsertdCas9-10xSunTag
UseTagsExpressionMammalianMutationPromoterchicken beta-actin promoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDragon-empty
Plasmid#155018PurposeEmpty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.DepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEYFP_C1_FANCM
Plasmid#221384PurposeExpresses FANCM in mammalian cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorInsertp1k-PEX1-2GFP (PEX1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-SIINFEKL
Plasmid#174601Purposeexpresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitopeDepositorInsertmembrane bound CD19, SIINFEKL (ovalbumin epitope)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM5
Plasmid#133635PurposeIntegration of CHS5-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorInsertp1k-CHS5-GFP (CHS5 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM10
Plasmid#133639PurposeIntegration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.DepositorInsertp1k-Vps4-GFP (VPS4 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDragon-DTA
Plasmid#155017PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to attenuated diphtheria toxin (tox176) in the presence of Cre and (r)tTA activityDepositorInsertDTA
UseMouse TargetingTagsExpressionMutationtox176PromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-EMC1-2GFP (EMC1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV U6-sgRNA UbC-tdTomato
Plasmid#106949PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-ANP1-GFP (ANP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorInsertSEC7-2GFP (SEC7 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM25
Plasmid#133672PurposeIntegration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.DepositorInsertp1k-MSP1-2GFP (MSP1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-LLO-SIINFEKL
Plasmid#174598Purposeexpresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopesDepositorInsertmembrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtMIR390a-NbSu-2
Plasmid#213400PurposePlant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursorDepositorInsertArabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZJOM30
Plasmid#133673PurposeIntegration of ERG6-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on ER and lipid droplets.DepositorInsertp1k-ERG6-GFP (ERG6 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-MBD1 R44Q-14-mVenusC
Plasmid#191394PurposeR44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenusDepositorInsertpcDNA3 Flag-MBD1 R44Q-14-mVenusC
UseTags3xFLAGExpressionMammalianMutationR44QPromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM2
Plasmid#133633PurposeIntegration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorInsertp1k-VRG4-GFP (VRG4 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-MPHOSPH8 Chromo
Plasmid#59694PurposeContains histone modification interacting domain (MPHOSPH8 Chromo)DepositorInsertMPHOSPH8 Chromo (MPHOSPH8 Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-2xFLAG-Atoh1ER-DsRed
Plasmid#101735PurposeExpresses mouse Atoh1 fused to ER and DsRedDepositorInsertAtoh1 (Atoh1 Mouse)
UseTags2x FLAG, DsRed, and ERExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM8
Plasmid#133671PurposeIntegration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.DepositorInsertp1k-SNF7-GFP (SNF7 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP-IFP2.0C
Plasmid#215752PurposeExpresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD-IFP2.0C
Plasmid#215754PurposeExpresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mVenusC-14-HP1b Chromo W42A
Plasmid#191399PurposeW42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenusDepositorInsertFLAG-mVenusC-14-HP1b Chromo W42A
UseTags3xFLAGExpressionMammalianMutationW42APromoterCMVAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Hic1 GFP
Plasmid#106953PurposeLentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.DepositorInsertanti-Hic1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Yeast Secrete and Detect
Plasmid Kit#1000000166PurposeYeast Golden Gate cloning for protein secretion in Saccharomyces cerevisiae and Pichia pastoris. Methanol utilization (MUT) promoters, signal peptides, C-terminal tags, episomal and integrative.DepositorAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe_Hyg_cOVA_T2A_mStrawberry
Plasmid#161737PurposeExpression of cOVA and mStrawberryDepositorInsertcytoplasmic ovalbumin
UseRetroviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
B52 (empty plasmid backbone to express 2 sgRNAs)
Plasmid#100708PurposeEmpty plasmid backbone to express 2 sgRNAs (use BbsI and BsmBI for cloning)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 promotersAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPTF PHD and Bromodomain
Plasmid#92101PurposeExpresses GST fusion PHD and Bromodomain from BPTF proteinDepositorInsertPHD and Bromodomain (BPTF Human)
UseTagsGST TagExpressionBacterialMutationPromotertac promoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18
Plasmid#131670PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.DepositorInserte18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
2xHP1bCD_W42A-IFP2.0C
Plasmid#215751PurposeExpresses binding-deficient W42A mutant of 2xHP1beta chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K9me2/3 detection in BiAD sensorsDepositorInsert2xHP1bCD_W42A-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
hGAPDH-rLuc
Plasmid#82479Purposereporter of GAPDH expressionDepositorInsertGAPDH promoter (GAPDH Human)
UseLuciferaseTagsExpressionBacterialMutationPromoterhGAPDHAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECFP_C1_NSD2 FL
Plasmid#221382PurposeExpresses NSD2 in mammalian cellsDepositorInsertHistone-lysine N-methyltransferase NSD2 (NSD2 Human)
UseTagsExpressionMammalianMutationL237R mutation in ECFP (Please see depositor comm…PromoterCMVAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe_Zeo_cOVA_T2A_mStrawberry
Plasmid#161736PurposeExpression of cOVA and mStrawberryDepositorInsertcytoplasmic ovalbumin
UseRetroviralTagsmStrawberryExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C
Plasmid#184410Purposebacterial expression of TTRDepositorInsertTTR (TTR Human)
UseTagsExpressionBacterialMutationC10S, S85CPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_FANCM
Plasmid#221379PurposeExpresses FANCM in bacterial cellsDepositorInsertFanconi anemia group M protein (FANCM Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-Tyr
Plasmid#135616PurposeDonor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locusDepositorInsertEcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV U6-sgRNA UbC-GFP
Plasmid#106948PurposesgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP markerDepositorInsertsgRNA BsmBI/Esp3I cloning site
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo
Plasmid#191397PurposesgRNA targeting alpha-satellites on human chromosome 9DepositorInsertsgRNA targeting alpha satellites on human chromosome 9
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_ATRX
Plasmid#221377PurposeExpresses ATRX in bacterial cellsDepositorInsertTranscriptional regulator ATRX (ATRX Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #1 GFP
Plasmid#106950PurposeLentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only