We narrowed to 1,296 results for: AP2;

1. pminiT-Halo-MED1 donor

   Plasmid

   #197868

   Purpose

   Donor plasmid used for building Halo-MED1 knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   CRSP210 (Med1 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Halotag

   Expression

   Mutation

   Promoter

   NA

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO-shcIAP1-B

   Plasmid

   #44131

   Depositor

   William Hahn

   Article

   Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

   Insert

   c-IAP1 (BIRC2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA FLAG mTOR ST

   Plasmid

   #22999

   Depositor

   Jing Huang

   Article

   Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

   Insert

   mTOR (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2035T

   Promoter

   Available since

   May 18, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-Flag-YAP-S61A

   Plasmid

   #33087

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S61A

   Promoter

   CMV

   Available since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

5. PX330_MED13_iso1_1

   Plasmid

   #135751

   Purpose

   Encodes gRNA for 3' target of human MED13_iso1

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   MED13_iso1 gRNA (MED13 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-FRB-W2027F

   Plasmid

   #73341

   Purpose

   Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR

   Depositor

   Jie Chen

   Article

   Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

   Insert

   FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
   

   Use

   Tags

   GST-tagged

   Expression

   Bacterial

   Mutation

   FRB domain only aa2015-2114; W2027F

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-Flag-YAP-S381A

   Plasmid

   #33090

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   YAP (YAP1 )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S381A

   Promoter

   CMV

   Available since

   Feb. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA Flag Yap1 Y357E

   Plasmid

   #18883

   Depositor

   Yosef Shaul

   Article

   Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

   Insert

   Yap1 Y357E (YAP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Y357E

   Promoter

   Available since

   Aug. 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3 Flag MKK7g1(K165A)

   Plasmid

   #14675

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7g1(K165A) (Map2k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Lys-165Ala

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-2T FRB Y2105A

    Plasmid

    #68726

    Purpose

    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    

    Use

    Tags

    GST-tagged

    Expression

    Bacterial

    Mutation

    FRB domain only aa2015-2114; Y2105A

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. HA SEK AL pcDNA3.1 zeo (+) - SEK1 (A-L)

    Plasmid

    #24653

    Depositor

    Jim Woodgett

    Article

    Yan et al Nature. . 372(6508):798-800.

    Insert

    SEK1 (MAP2K4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Ser220Ala, Thr224Leu

    Promoter

    Available since

    June 21, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCEP-WAF1-ES

    Plasmid

    #16449

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

    Insert

    WAF1 stop codon mut (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    stop codon at amino acid 49 instead of tryptophan…

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-Flag-YAP-H122L

    Plasmid

    #33086

    Depositor

    Kunliang Guan

    Article

    Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

    Insert

    YAP (YAP1 )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    H122L

    Promoter

    CMV

    Available since

    March 21, 2012

    Availability

    Academic Institutions and Nonprofits only

14. TurboID-MEK1

    Plasmid

    #226169

    Purpose

    Used to transiently express a TurboID fusion of the protein kinase MEK1.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    Myc-TurboID-MEK1 (MAP2K1 Human)
    

    Use

    Tags

    Myc-TurboID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGex-6p2_FANCM_K819R

    Plasmid

    #221380

    Purpose

    Expresses FANCM_K819R in bacterial cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Fanconi anemia group M protein (FANCM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Changed lysine 819 to arginine

    Promoter

    tac

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM166B

    Plasmid

    #218123

    Purpose

    For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    rpoZ

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA

    Plasmid

    #214614

    Purpose

    adapter for RTK split TEV recruitment assays

    Depositor

    Michael Wehr

    Article

    Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

    Insert

    3xSH2 domains of GRB2-CTEV (GRB2 Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pminiT-mEmerald-MED1 donor

    Plasmid

    #197867

    Purpose

    Donor plasmid used for building mEmerald-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTij_sg_mmu_Med1_N_terminal

    Plasmid

    #197870

    Purpose

    CRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p8319 pDONR ATG-YAP1 WT-Stop

    Plasmid

    #184524

    Purpose

    Subcloning YAP1 WT

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    YAP1 WT (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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