We narrowed to 32,487 results for: Eng

1. pDSM-cd-Pfba1p-ACO-Tgpm1

   Plasmid

   #127731

   Purpose

   Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   aconitase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Pfba1

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDSM-cd-Pykt6-ACO-Tprm9

   Plasmid

   #127732

   Purpose

   Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   aconitase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Pykt6

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDSM-cd-Ptef1-ACO-Tyol036w

   Plasmid

   #127733

   Purpose

   Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   aconitase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Ptef1

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDSM-ef-Ptef1-CSC-Tpdc1

   Plasmid

   #127739

   Purpose

   Yeast pathway position 6. CSC transcription unit with the TEF1 promoter and PDC1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   citrate synthase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Pfba1

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDSM-ef-Pzuo1-CSC-Tvma16

   Plasmid

   #127740

   Purpose

   Yeast pathway position 6. CSC transcription unit with the ZUO1 promoter and VMA16 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   citrate synthase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Pzuo1

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDSM-ef-Ppfy1-CSC-Taip1

   Plasmid

   #127741

   Purpose

   Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   citrate synthase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Ppfy1

   Availability

   Academic Institutions and Nonprofits only

   

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7. CYN2/150 (E. coli Entner-Doudoroff v11)

   Bacterial Strain

   #71420

   Purpose

   This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant

   Depositor

   Howard Salis

   Article

   Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

8. CYN1 (E. coli Entner-Doudoroff v1)

   Bacterial Strain

   #71421

   Purpose

   This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway (first version). The module also contains a Cm resistance marker.

   Depositor

   Howard Salis

   Article

   Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10

   Bacterial Resistance

   Chloramphenicol

   Available Since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Keasling-2337

   Plasmid

   #100168

   Purpose

   Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

   Depositor

   Jay Keasling

   Article

   Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

   Insert

   lacIq-Ptrc-ADS
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS1C

    Plasmid

    #55168

    Purpose

    Empty vector, integration at amyE, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Available Since

    Aug. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pREDCas9

    Plasmid

    #71541

    Purpose

    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Inserts

    Cas9
    lambda red genes
    plasmid curing system
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. evoBxb1

    Plasmid

    #222338

    Purpose

    Plasmid for expressing evolved Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS2307-Tier1-2xcHS4-PPGK-NLuc_TB

    Plasmid

    #169550

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a tandem repeat of a 5' cHS4 insulator sequence (2xcHS4-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cHS4-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-mCherry

    Plasmid

    #108685

    Purpose

    Strong CAG promoter with mCherry expression for transfection efficacy screening

    Depositor

    Jordan Green

    Article

    Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Linear-GFP

    Plasmid

    #226258

    Purpose

    Production of linear RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Budding Yeast, Human)
    

    Expression

    Bacterial

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Circular-oc-EMCV-GFP

    Plasmid

    #226261

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. amilCP chromoprotein

    Plasmid

    #117847

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. PxylA

    Plasmid

    #55178

    Purpose

    Xylose-inducible promoter

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Insert

    xylA promoter
    

    Use

    Synthetic Biology; Bacillus biobrick box

    Promoter

    xylA

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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