Addgene

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NOVA1_Full-length_pGEX

Plasmid

#135147

Purpose

Recombinant expression of NOVA1 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

NOVA1 (NOVA1 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

Plasmid

#159658

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

Plasmid

#159657

Purpose

CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

Depositor

Joseph Brzezinski

Article

Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

Insert

hSpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Ef1a

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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PCBP4_3xKH_pGEX

Plasmid

#135156

Purpose

Recombinant expression of PCBP4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

PCBP4 (PCBP4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-N23PP/G51PEKN

Plasmid

#118583

Purpose

Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

Depositor

Stephen Benkovic

Article

Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

Insert

DHFR

Use

Tags

Expression

Bacterial

Mutation

N23 mutated to P23, G51 mutated to P51, a Pro res…

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTV-mHdac1-tdiRFP670-PEST2

Plasmid

#122351

Purpose

Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Hdac1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Met-Eif4a1-tdiRFP670-PEST

Plasmid

#122371

Purpose

Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST-2A with homology arms for Eif4a1

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-Eif4a1-L

Plasmid

#122346

Purpose

Expresses sgRNA targeting mouse Eif4a1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Eif4a1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[1-20]-N-int

Plasmid

#199210

Purpose

For ubiquitous expression of split-intein Gal4-N-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein Gal4[1-20]-N-int

Use

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTub-split-intein-Gal4[21-881]-C-int

Plasmid

#199211

Purpose

For ubiquitous expression of split-intein Gal4-C-int component under control of the tubulin promoter

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[21-881]-C-int

Use

Tags

Expression

Mutation

Promoter

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP103: pTarget(CyCAST)

Plasmid

#200849

Purpose

Target plasmid containing CyPSP1 and attachement site for CyCAST.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Inserts

PAM for CyCAST + CyPSP1
tRNA-Leu

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

Plasmid

#200845

Purpose

Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP122: pHelper(CyCAST)_Shuffled-spacer

Plasmid

#200841

Purpose

Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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MFE23-ZZ

Plasmid

#223553

Purpose

MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.

Depositor

Jean Gariepy

Article

Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6

Insert

pET302-MFE23-ZZ

Use

Tags

HA tag and HIS tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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p202-Syt1-C2A-wt

Plasmid

#201553

Purpose

Expresses wild-type Octopus synaptotagmin-1 C2A domain in E. coli

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Synaptotagmin-1 C2A domain (partial)

Use

Tags

Expression

Bacterial

Mutation

Codon-optimized for E. coli

Promoter

RBS

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA pCAGGS

Plasmid

#206052

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/B-actin

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-IEo

Plasmid

#201992

Purpose

AAV vector mediating inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Depositor

Wei Xu

Article

Du et al Cell Rep Methods. 2023 Jun 16;3(6):10050

Insert

Inducible expression of codon optimized immediate early gene IE180 of pseudorabies virus

Use

AAV

Tags

Expression

Mutation

Promoter

Tight TRE

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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