Skip to main content
Addgene
Showing: 861 - 880 of 5507 results

  11. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid
    #191093
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B and mScarlet
    Use
    AAV
    Tags
    H2B-V5-mScarlet
    Expression
    Mammalian
    Mutation
    The fusion protein was optimized to the Human cod…
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191093-plasmid-map-sequence-id-380126

    Enlarge

  12. pAAV2-CAG-mGreenLantern-KASH

    Plasmid
    #191094
    Purpose
    To express a bright monomeric green FP to label the eucaryotic nuclear envelope. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    mGreenLantern-KASH
    Use
    AAV and Synthetic Biology
    Tags
    mGreenLantern fused to the KASH domain of Nesprin…
    Expression
    Mammalian
    Mutation
    Optimizzation was done using the Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191094-plasmid-map-sequence-id-380125

    Enlarge

  15. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid
    #145145
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Use
    Tags
    8his tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression.
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145145-plasmid-map-sequence-id-324821

    Enlarge

  16. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

    Enlarge

  17. pAAV2-CAG-3xNLS-vsfGFP-0

    Plasmid
    #191097
    Purpose
    To express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-vsfGFP-0
    Use
    AAV
    Tags
    Green fluorescent protein bound to enhancer nanob…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191097-plasmid-map-sequence-id-380121

    Enlarge

  18. pAAV2-CAG-3xNLS-mScarlet

    Plasmid
    #191098
    Purpose
    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-mScarlet
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191098-plasmid-map-sequence-id-380124

    Enlarge

  19. pAAV-CAG-H2BmGreenLantern

    Plasmid
    #177332
    Purpose
    To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.
    Depositor
    Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    H2B (Hist1h2bq Rat)
    Use
    AAV
    Tags
    mGreenLantern
    Expression
    Mammalian
    Mutation
    This fusion protein was optimized to the Human co…
    Promoter
    CAG
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177332-plasmid-map-sequence-id-353323

    Enlarge

  20. pAAV2-CAG-3xNLS-AausFP1

    Plasmid
    #191096
    Purpose
    To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods
    Depositor
    Murray Blackmore, Pantelis Tsoulfas
    Article
    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:
    Insert
    3xNLS-AausFP1
    Use
    AAV and Synthetic Biology
    Tags
    Three repeats of the nuclear localizzation seque…
    Expression
    Mammalian
    Mutation
    Optimized to Human codon usage
    Promoter
    CMV enhancer and CAG
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    191096-plasmid-map-sequence-id-380123

    Enlarge

Showing: 861 - 880 of 5507 results