We narrowed to 1,143 results for: fret

1. pAD/CMV/V5-DEST-Cdc42-2G

   Plasmid

   #68812

   Purpose

   FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

   Depositor

   Olivier Pertz

   Article

   Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

   Insert

   Cdc42 activation reporter
   

   Use

   Adenoviral

   Tags

   His and Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCX-ThPDE5VV

   Plasmid

   #134778

   Purpose

   cGMP FRET biosensor

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

   Insert

   ThPDE5VV
   

   Tags

   cpVenus-Venus and mTurquoise2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-ER-AKAR4

   Plasmid

   #64733

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   AKAR4
   

   Tags

   Cerulean, N-terminal 27 amino acids from CYP450, …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. aCKAR

   Plasmid

   #124427

   Purpose

   FRET-based reporter for monitoring aPKC activity in cells

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al Sci Signal. 2019 Jan 1;12(562). pii: 12/

   Insert

   aCKAR
   

   Tags

   mCFP and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-ER-CaNAR2

   Plasmid

   #64732

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CYP450-CaNAR2
   

   Tags

   Cerulean3, N-terminal 27 amino acids from CYP450,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-Mito-CaNAR2

   Plasmid

   #64731

   Purpose

   Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Tags

   Cerulean3, N-terminal 30 amino acids from DAKAP1,…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-ICUE3-NLS

   Plasmid

   #138218

   Purpose

   Nuclear-targeted FRET-based cAMP indicator.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   ICUE3-NLS
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-ABKAR

   Plasmid

   #140051

   Purpose

   Genetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.

   Depositor

   Jin Zhang

   Article

   Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

   Insert

   ABKAR
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-AktAR-Kras

   Plasmid

   #123357

   Purpose

   FRET-based biosensor for monitoring Akt activity in non-raft plasma membrane regions.

   Depositor

   Jin Zhang

   Article

   Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

   Insert

   AktAR-Kras
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1(+)-Mito-CaNARi

    Plasmid

    #64851

    Purpose

    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNARi
    

    Tags

    Cerulean, N-terminal 30 amino acids from DAKAP1, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDNA3-Cerulean-FHA1-NES

    Plasmid

    #138202

    Purpose

    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    Cerulean-FHA1-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pECFP-Rac1Y40C

    Plasmid

    #105293

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1(+)-ER-BSCaM2

    Plasmid

    #64855

    Purpose

    Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-BSCaM2
    

    Tags

    CFP, N-terminal 27 amino acids from CYP450, and Y…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-ER-CaNARi

    Plasmid

    #64852

    Purpose

    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CYP450-CaNARi
    

    Tags

    Cerulean, N-terminal 27 amino acids from CYP450, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pECFP-Cdc42-Y40C

    Plasmid

    #105295

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPBbsr2-RaichuEV-Rac1HRasCT

    Plasmid

    #214818

    Purpose

    Encoding a Förster resonance energy transfer (FRET) biosensor for Rac1 activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

    Insert

    RaichuEV-Rac1HRasCT
    

    Tags

    HRasCAAX

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    July 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MCUb-mVenus-pLJM

    Plasmid

    #236789

    Purpose

    MCUB tagged with mVenus for FRET / MW change for stoichiometry

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB (MCUB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-PM-deltaCKAR

    Plasmid

    #222429

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Plasma membrane delta-selective C kinase activity reporter
    

    Tags

    CFP and YFP

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Nuc-deltaCKAR

    Plasmid

    #222430

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Nucleus delta-selective C kinase activity reporter
    

    Tags

    CFP and YFP

    Expression

    Mammalian

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Golgi-deltaCKAR

    Plasmid

    #222428

    Purpose

    FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

    Depositor

    Alexandra Newton

    Article

    Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

    Insert

    Golgi delta-selective C kinase activity reporter
    

    Tags

    CFP and YFP

    Expression

    Mammalian

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge