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Showing: 861 - 880 of 1127 results

  1. pcDNA3.1(+)-Mito-CaNARi

    Plasmid
    #64851
    Purpose
    Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CaNARi
    Use
    Tags
    Cerulean, N-terminal 30 amino acids from DAKAP1, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64851-plasmid-map-sequence-id-109676

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  2. pDNA3-Cerulean-FHA1-NES

    Plasmid
    #138202
    Purpose
    Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    Cerulean-FHA1-NES
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    138202-plasmid-map-sequence-id-307221

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  3. pcDNA3.1(+)-ER-BSCaM2

    Plasmid
    #64855
    Purpose
    Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-BSCaM2
    Use
    Tags
    CFP, N-terminal 27 amino acids from CYP450, and Y…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64855-plasmid-map-sequence-id-109681

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  4. pcDNA3.1(+)-ER-CaNARi

    Plasmid
    #64852
    Purpose
    Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-CaNARi
    Use
    Tags
    Cerulean, N-terminal 27 amino acids from CYP450, …
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64852-plasmid-map-sequence-id-109677

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  10. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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  11. pBAD-MTX-u51_Snifit

    Plasmid
    #119046
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u51_Snifit
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    51TAG R44L H45Q
    Promoter
    pBAD
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119046-plasmid-map-sequence-id-229503

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  12. pBAD-MTX-u23_Snifit

    Plasmid
    #119044
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Article
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u23_Snifit
    Use
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    23TAG R44L H45Q
    Promoter
    pBAD
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119044-plasmid-map-sequence-id-229496

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  20. pcDNA3-Lyn-PlcR

    Plasmid
    #181830
    Purpose
    Genetically encoded FRET-based sensor for monitoring plasma membrane PI(4,5)P2 dynamics in living cells.
    Depositor
    Jin Zhang
    Article
    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.
    Insert
    Lyn-PlcR
    Use
    Tags
    ECFP, EYFP, and N-terminal targeting sequence fro…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181830-plasmid-map-sequence-id-364215

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Showing: 861 - 880 of 1127 results