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We narrowed to 11,227 results for: hal

Showing: 861 - 880 of 11227 results

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pF(UG) hSyn SYT7alpha-HaloTag P2A PM-msGFP

Plasmid

#179717

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag and PM-targeted msGFP

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Halo-CD4-bla

Plasmid

#189982

Purpose

Donor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Halo-CD4-bla

Plasmid

#189986

Purpose

Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (L115R)- HaloTag

Plasmid

#205712

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag and XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-V5-HisB-ObKHC-K282R-HaloTag

Plasmid

#201552

Purpose

Expresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cells

Depositor

Joshua Rosenthal

Article

Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

Insert

Kinesin-1

Use

Tags

HaloTag

Expression

Insect

Mutation

changed lysine 282 to arginine (numbering based o…

Promoter

Ac5

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.HALO-KIF3A tail 419-702

Plasmid

#193924

Purpose

Mammalian expression of Halo-tagged Kif 3A tail 419-702

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3A (KIF3A Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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SiRES COR1C ACT-DCC-Halo

Plasmid

#195148

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

mutations to make si RNA resistant and remove act…

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-BRAF-REG-T241P-Halo

Plasmid

#202549

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Threonine 241 mutated to proline

Promoter

CMV

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Spe4-Kozak-Halotag-RfA

Plasmid

#186377

Purpose

Destination vector for the expression of a fluorescent fusion of N-ter Halo tag and a protein of interest into embryos, by mRNA microinjection

Depositor

Patrick Lemaire

Type

Empty backbone

Use

Gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3B tail 391-747

Plasmid

#193929

Purpose

Mammalian expression of Halo-tagged Kif 3B tail 391-747

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3B (Kif3b Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPW3757 : CMVd3-ERmembrane-2xHaloTag-KKMP

Plasmid

#185679

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-2xHaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-H189AD194A-Halo

Plasmid

#195168

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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siRNA-resistant ABHD16A-S355A-Halo

Plasmid

#195169

Purpose

mammalian expression of siRNA-resistant mutant ABHD16A tagged with Halo tag

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Use

Tags

Halo tag

Expression

Mammalian

Mutation

Wobble mutations for siRNA resistance from L90 to…

Promoter

CMV

Available since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.Halo-KIF3C tail 411-796

Plasmid

#193934

Purpose

Mammalian expression of Halo-tagged Kif 3C tail 411-796

Depositor

Marvin Bentley

Article

Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

Insert

Kif 3C (Kif3c Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

Promoter

Chicken Beta actin

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4_HA-Halo-cmyc-GRM3 C127A

Plasmid

#140458

Purpose

Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A

Depositor

Erik Procko

Insert

GRM3 (GRM3 Human)

Use

Tags

Halo, Signal/leader sequence from influenza hemag…

Expression

Mammalian

Mutation

Cysteine 127 to alanine, Threonine 174 to alanine

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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PX552-mScn8a-HaloTag-V5-EF1-sfGFP

Plasmid

#182565

Purpose

For mouse Nav1.6 knockin with HaloTag-V5 at the C-terminal

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a gRNA and HaloTag-V5 donor DNA
sfGFP

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

EF1 and U6

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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Halo-WIPI2B H85E K88E I92E

Plasmid

#175033

Purpose

Expresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (H85E K88E I92E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Histadine 85 to Glutamate (CAC to GAG); Lysine 88…

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176134

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEntr1a-SspB-HaloTag-TRIO-DHPH1

Plasmid

#176133

Purpose

Heterodimerization with iLID, Rac1 and RhoG activation

Depositor

Dorus Gadella

Insert

TRIO (TRIO Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo G-Linker nsp1

Plasmid

#175426

Purpose

Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

G-linker nsp1

Use

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

G-linker in place of the RNase destabilization do…

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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