We narrowed to 6,093 results for: pcDNA 3.1

1. pcDNA3.1-Zeo-BFP

   Plasmid

   #214367

   Purpose

   Expression of the Blue Fluorescent Protein

   Depositor

   Bertrand Collet

   Article

   Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

   Insert

   BFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-UBE2E1 C131A in pcDNA3.1

   Plasmid

   #118214

   Purpose

   Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed cysteine 131 to alanine

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 MYC dHLH

   Plasmid

   #176047

   Purpose

   Expresses Myc Mutant (delta helix-hoop-helix) in Mammalian cells

   Depositor

   Linda Penn

   Article

   Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

   Insert

   Myc
   

   Expression

   Mammalian

   Mutation

   deleted amino acids 371-412

   Promoter

   CMV

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY111 (pcDNA3.1-huTsCpf1)

   Plasmid

   #92267

   Purpose

   Expression of humanized TsCpf1

   Depositor

   Feng Zhang

   Insert

   huTsCpf1
   

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_mGFP-FGFR3-K650Q

   Plasmid

   #191756

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1948A>C (p.K650Q) + silent mutation c.1938C&…

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-ccdB-Renilla

   Plasmid

   #87065

   Purpose

   Mammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Tags

   Renilla luciferase

   Expression

   Mammalian

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-TIE-V5/HIS

   Plasmid

   #205612

   Purpose

   Expression of human TIE receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TIE receptor tyrosine kinase (TIE1 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)-mCherry-p115

   Plasmid

   #181996

   Purpose

   Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.

   Depositor

   Jin Zhang

   Article

   Zhang et al PLoS Biol. 2020 Sep 3;18(9):e3000866. do

   Insert

   mCherry-p115 (ARHGEF1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CHRFAM7A-mCherry

   Plasmid

   #62635

   Purpose

   mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mCherry fused into M3-M4 loop

   Depositor

   Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRFAM7A (CHRFAM7A Human)
   

   Expression

   Mammalian

   Mutation

   mCherry inserted into M3-M4 loop

   Promoter

   CMV

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. CRT-GFP pcDNA3.1

    Plasmid

    #80978

    Purpose

    mammalian expression of CRT-GFP

    Depositor

    Peter Steyger

    Article

    Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

    Insert

    CRT (Calr Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    no KDEL

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

11. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY014 (pcDNA3.1-hMbCpf1)

    Plasmid

    #69986

    Purpose

    Expresses humanized MbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hMbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-N-Myc_DW164AA USP7

    Plasmid

    #131252

    Purpose

    Mammalian expression of N-terminally Myc-tagged DW164AA USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 aspa…

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+) mMff isoform 5

    Plasmid

    #44601

    Depositor

    David Chan

    Article

    Loson et al Mol Biol Cell. 2013 Jan 2.

    Insert

    mMff isoform 5 (Mff Mouse)
    

    Expression

    Mammalian

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.1-Flag-H2A K5-9R

    Plasmid

    #63562

    Purpose

    Expression of human H2A with K5R and K9R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 5 and Lysine 9 are now Arginine

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3.1 Bach1 S990A

    Plasmid

    #73643

    Purpose

    Mammalian expression of Bach1 S990A mutant (BRCA binding mutant).

    Depositor

    Sharon Cantor

    Insert

    Bach1 (BACH1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    S990A (BRCA binding mutant)

    Promoter

    CMV

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1_GEM22-Halo-FRB

    Plasmid

    #197059

    Purpose

    Expression of Mycobacterium hassiacum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-GoldenGate-VP64

    Plasmid

    #47389

    Purpose

    Expression plasmid for TALE proteins fused to VP64 activation domain compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FLAG, HA, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1_FRB-GEM23-Halo

    Plasmid

    #197060

    Purpose

    Expression of nanocage I3-01 Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    I3-01
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MAP3K5 (R256C)

    Plasmid

    #47105

    Purpose

    Expresses mutant (R256C) human MAP3K5 in mammalian cells

    Depositor

    Yardena Samuels

    Insert

    MAP3K5 (MAP3K5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 256 to cysteine

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only