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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,240 results for: spl

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Showing: 861 - 880 of 4240 results

  3. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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  4. pIND-hygro-HA-H3.3

    Plasmid
    #47981
    Purpose
    Ponasterone A-inducible expression of human HA tagged H3F3A
    Depositor
    Bing Zhu
    Publication
    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H3F3A (H3-3A Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    288 bp T to C synonymous mutation
    Promoter
    Minimal Heat Shock Promoter
    Available since
    Sept. 5, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. pCMV/MASC_(GLuc)_TOP1

    Plasmid
    #68439
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68439-plasmid-map-sequence-id-120217

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  10. tol2-CAG::ChR2-YFP

    Plasmid
    #59740
    Purpose
    Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)
    Depositor
    Ivo Lieberam
    Publication
    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Channelrhodopsin-2
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    CAG
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. CAG sHRPa-FRB-pre mGRASP

    Plasmid
    #73145
    Purpose
    Large fragment of split HRP and FRB fused to the extracellular terminus of the "pre mGRASP" scaffold published by Magee, in which the extracellular domain of NRX is replaced by portions of CD4.
    Depositor
    Alice Ting
    Publication
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sHRPa-FRB-pre mGRASP
    Tags
    Flag tag
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73145-plasmid-map-sequence-id-134517

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  15. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

    Plasmid
    #137830
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Publication
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Mutated Leucine 71 to Lysine in CTEVp fragment
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137830-plasmid-map-sequence-id-311956

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  18. pComb3HTT

    Plasmid
    #63900
    Purpose
    Backbone of pComb3H containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control.
    Depositor
    Carlos Barbas
    Publication
    Barbas et al Methods, A companion to Methods in Enzym
    Insert
    TT Fab
    Use
    Phage display
    Tags
    geneIII
    Expression
    Bacterial
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63900-plasmid-map-sequence-id-119142

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Showing: 861 - 880 of 4240 results