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pcDNA3.1(+)_Nav1.6WT-HA

Plasmid

#239762

Purpose

Expresses mouse Nav1.6WT with a C-terminal HA tag in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Publication

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Scn8a (Scn8a Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP5 Cys146Ala

Plasmid

#154028

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-AILR-LplA-ER

Plasmid

#42574

Depositor

Alice Ting

Publication

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LplA(AILR) with ER-retention sequence KDEL

Tags

FLAG

Mutation

Compared to wild-type LplA: tryptophan 37 to alan…

Promoter

CAG

Available since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
plvx-tetO-GFP

Plasmid

#191560

Purpose

Inducible expression of GFP

Depositor

Alon Herschhorn, Joseph Sodroski

Publication

Herschhorn et al PLoS One. 2011;6(11):e26731. doi: 10.137

Insert

gfp

Use

Lentiviral

Mutation

None

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292

Plasmid

#129672

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

AaCas12b is rice codon optimized

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p15A_SpyCas9_CmR

Plasmid

#119162

Purpose

Expresses S. pyogenes Cas9

Depositor

Vincent Noireaux

Publication

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 from S. pyogenes (NEWENTRY )

Use

Crispr

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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gS gag LV

Plasmid

#12327

Depositor

Inder Verma

Publication

Kootstra et al Proc Natl Acad Sci U S A. 2003 Feb 4. 10

Insert

gag/pol

Use

Lentiviral

Expression

Mammalian

Mutation

The CypA binding region of the WT gag was replace…

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pCI-neoBeta2mcherry

Plasmid

#45097

Depositor

Henry Lester

Publication

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nAChR beta2 mcherry (Chrnb2 Mouse)

Tags

has c-myc tag

Expression

Mammalian

Mutation

β2 was cloned into pCI-neo at the restriction sit…

Promoter

CMV

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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scAAV-hSyn-Cre

Plasmid

#138253

Purpose

A self-complementary AAV vector expresses Cre recombinase from hSyn promoter.

Depositor

Li Zhang

Publication

Zingg et al J Neurosci. 2020 Apr 15;40(16):3250-3267

Insert

Cre recombinase

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Violet

Plasmid

#160447

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Violet chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Violet

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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YWHAH

Plasmid

#38814

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

YWHAH (Ywhah Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p177bp-LacOx162-Hyg

Plasmid

#210215

Purpose

Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

162xLacO repeats derived from: PMID: 12864855.

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MAC-SARS-CoV-2 S

Plasmid

#158371

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Publication

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 S (S )

Tags

MAC-tag

Expression

Mammalian

Available since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBW300ParsR-Amp32T

Plasmid

#78652

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Publication

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pR-mKate2

Plasmid

#228610

Purpose

Mammalian expression of mKate2, for MuSIC barcode construction

Depositor

Marc Birtwistle

Publication

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mKate2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Publication

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gfp11::rps-18 (rps-18 Nematode, Synthetic)

Use

Gateway Cloning

Tags

GFP11

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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