We narrowed to 6,363 results for: wus

1. pCMV-AsCas12f1-v5.1+T1-empty

   Plasmid

   #204977

   Purpose

   AsCas12f1-v5.1+T1-based Human cell genome editing

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nature Catalysis (2023) 6, 695–709

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N70Q/K103R/A104R/S118A/D364R

   Promoter

   PCMV,U6

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EGFR-L858R-nick sgRNA

   Plasmid

   #214097

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-EGFR-T790M-nick sgRNA

   Plasmid

   #214098

   Purpose

   Lentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR T790M nicking sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_tKiMBI-T2A-caMEK

   Plasmid

   #199578

   Purpose

   Expresses tKiMBI and caMEK in an AAV vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator
   constitutively active MEK
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-FRISZ

   Plasmid

   #176887

   Purpose

   Far-red fluorescent zinc indicator FRISZ in pDisplay

   Depositor

   Huiwang Ai

   Article

   Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

   Insert

   FRISZ
   

   Use

   Tags

   pDisplay backbone elements for export and membran…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin

   Plasmid

   #199585

   Purpose

   Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vector

   Depositor

   Michael Lin

   Article

   Wu et al ACS Cent Sci. 2023 Mar 20;9(4):719-732.

   Inserts

   ERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
   AkaLuc
   Blasticidin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST

   Plasmid

   #194386

   Purpose

   Gateway Destination vector for GPCR split TEV assays with V2R tail

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hyper7 in pT3TS-Dest

   Plasmid

   #194296

   Purpose

   In vitro transcription of the hydrogen peroxide sensor Hyper7 from the T3 promoter. Parton lab clone KRL

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   Hyper7
   

   Use

   In vitro transcription of mrna

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

   Plasmid

   #172226

   Purpose

   Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposon

   Tags

   3XFLAG, DD-domain, GS-linker, and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. plKO.1-DNAJC5-ShRNA

    Plasmid

    #205729

    Purpose

    Knockdown of DNAJC5

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    shRNA targeting DNAJC5 (DNAJC5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-DNAJC5 (WT)-HaloTag

    Plasmid

    #205710

    Purpose

    Mammalian expression of DNAJC5 (WT)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWay21-MKP-1L16A/L17A

    Plasmid

    #13478

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1L16A/L17A (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Changed Leucine 16 to Alanine; changed Leucine 1…

    Promoter

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pWay21-MKP-1DCH2AB

    Plasmid

    #13472

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1DCH2AB (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of MKP-1 CH2AB; contains aa 137-367

    Promoter

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pWay21-MKP-1DCH2A

    Plasmid

    #13470

    Depositor

    Anton Bennett

    Article

    Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

    Insert

    MKP-1DCH2A (Dusp1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of MKP-1 CH2A; contains aa 47-367

    Promoter

    Available Since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pGL3-Myc-rDMR

    Plasmid

    #235694

    Purpose

    Measure the activity of the reduced differentially methylated region (rDMR) of Myc with luciferase assay

    Depositor

    Duojia Pan

    Article

    Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

    Insert

    Myc (Myc Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLei-sfGFP-V2*D134*Y152*

    Plasmid

    #191112

    Purpose

    An E. coli sfGFP reporter with two TAG at positions 2 & 134 & 152

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    superfolder green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    changing V2 & D134 & Y152 to TAG

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-EcYRS-EGFP*

    Plasmid

    #191118

    Purpose

    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter for zebrafish

    Depositor

    Han Xiao

    Article

    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

    Insert

    Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-KRAS-G121C/G13A-nick sgRNA

    Plasmid

    #214099

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce KRAS G12C or G13A mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    KRAS G12C or G13A nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRX-HA-hSTING (S366A)

    Plasmid

    #214151

    Purpose

    Retroviral transduction of STING

    Depositor

    Nan Yan

    Article

    Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

    Insert

    STING (S366A) (STING1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    S366A

    Promoter

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-DNAJC5-L115R

    Plasmid

    #205708

    Purpose

    Mammalian expression of DNAJC5 L115R

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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