We narrowed to 24,758 results for: BASE
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Plasmid#173003Purposefirefly luciferase reporter vector with 18x clustered TEAD binding sitesDepositorInsert18xTEAD-luc2
UseAAV and LuciferaseExpressionMammalianAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SpdCas9-LBiT_Strep-Tag-II
Plasmid#198751PurposeExpresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferaseDepositorInsertSpdCas9 - LargeBiT
TagsStrep-tag IIExpressionBacterialMutationD10A, H840A (nuclease inactivating mutations in &…PromoterT7Available SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Cas9-pA-Cry-GFP
Plasmid#168239Purpose"neutrophil specific cas9 expression in zebrafish with green lens"DepositorInsertCas9
UseZebrafish expressionAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
TagsMyc tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP_cSuMMVp
Plasmid#119182PurposeExpresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP)DepositorInsertsplit cSuMMVp in fusion with FKBP
TagsHA tagExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL8 lenti-D10Acas9-3xflag-blast
Plasmid#112134Purposelenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)DepositorInsertD10Acas9-3xflag-blast
UseCRISPR and LentiviralExpressionMammalianMutationD10A mutant in Cas9PromoterEF1aAvailable SinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 GW-NL-myc
Plasmid#113447PurposeNanoLuc Gateway shuttle vector for C-terminal fusionsDepositorTypeEmpty backboneUseLuciferase; Gateway shuttle vectorTagsNanoLuc-cmycExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1RA-P2A-Puro
Plasmid#110853PurposeLentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_Actb NHEJ donor
Plasmid#97316PurposeNHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.DepositorInsertActb NHEJ donor
UseAAV and Mouse TargetingExpressionMammalianAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [M1_n-1] (GB1210)
Plasmid#75411PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [M1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pESC-MET
Plasmid#222482PurposeCo-expression of two genes in yeast with FLAG and MYC tags and MET15 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-LYS
Plasmid#222483PurposeCo-expression of two genes in yeast with FLAG and MYC tags and LYS2 selectionDepositorTypeEmpty backboneTagsFLAG and MycExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-ADE (V5 and HA)
Plasmid#222489PurposeCo-expression of two genes in yeast with V5 and HA tags and ADE2 selectionDepositorTypeEmpty backboneTagsHA and V5ExpressionYeastAvailable SinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fluorescent reporter for MOBE1-2
Plasmid#219945PurposeMammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2DepositorInsertCMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
UseCRISPRExpressionMammalianMutationEGFP(A111V/L202S)PromoterCMV, U6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
apt-CBE-3'end
Plasmid#219939PurposeMammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP markerDepositorInsertMCP_32-aa_evo-rA1_2xUGI
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
nCas9-NG_P2A-mCherry
Plasmid#219934PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-nCas9(D10A)-NG_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationnCas9(D10A)PromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Tgl3-4V5-APEX2-URA
Plasmid#207066PurposeExpressing Tgl3-4V5-APEX2. Marker for lipid droplets.DepositorInsertTgl3
Tags4xV5-APEX2ExpressionYeastPromoterpADH3Available SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-PM-TurboID
Plasmid#209395PurposeTransiently expressing TurboID fused with a plasma menbrane targeting peptide in plantaDepositorInsertPM-3XHA-TurbOID
ExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-Cre-PGK-Puro
Plasmid#207742PurposeCo-expression of the Cre recombinase and a puro selectable markerDepositorInsertCre-PGK-Puro
UseCre/LoxExpressionMammalianPromoterEF1alphaAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH1-shOPA1-pSyn-tdtomato
Plasmid#206316PurposeKnocking-down OPA1 in mouse cellsDepositorInsertshRNA against mouse OPA1
UseLentiviralExpressionMammalianAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only