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[pSK497] pLJC5 Flag-Depdc5(AB)

Plasmid

#109341

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant AB

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α1.1-SomArchon-GFP

Plasmid

#153531

Purpose

AAV-mediated expression of SomArchon-GFP under the EF1α1.1 promoter.

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-GFP

Use

AAV

Tags

ER2, GFP, and KGC

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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C-His-GvpC-TEV

Plasmid

#153296

Purpose

Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

TEV-sensitive Gas vesicle protein C (his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

1) 21 bp TEV recognition and cleavage site flanke…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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N-His-GvpC-ssrA

Plasmid

#153297

Purpose

Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)

Tags

His-tag and ssrA tag

Expression

Bacterial

Mutation

1) N-terminal 6xHis tag flanked by 1x glycine res…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-Rpb1-linker

Plasmid

#138468

Purpose

Expresses GST-tagged Rpb1 linker in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

Rpb1 (POLR2A Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1460-1585

Promoter

tac

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pKD251

Plasmid

#124714

Purpose

Expression of torS under Ptac and torT under PD59_luxR

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torS (torS )
torT (torS )
lacI (lacI )
luxR

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCR1110

Plasmid

#111096

Purpose

Expresses BLM_+_91260645.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

BLM gRNA (BLM Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1322

Plasmid

#111109

Purpose

Expresses FANCL_+_58468435.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCL gRNA (FANCL Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1271

Plasmid

#111106

Purpose

Expresses FANCI_-_89787239.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCI gRNA (FANCI Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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AcrIIA5-E76-LOV-C450A-K488N(M478)

Plasmid

#250778

Purpose

Expresses AcrIIA5-E76-AsLOV2 with C450A and K488N mutation from a CMV promoter. For use in mammalian cells

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

AcrIIA5 with an AsLOV2 insertion behind E76 and C450A and K488N mutation

Use

CRISPR

Expression

Mammalian

Mutation

C450A, K488N

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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SpyCas9-E566-LOV-C450A-D432V(M519)

Plasmid

#250779

Purpose

Potent temperature-deactivated SpyCas9

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

SpyCas9 with an AsLOV2 insertion behind E566 and C450A and D432V mutation

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

C450A, D432V

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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AraC-S170-LOV-C450A-K488N(M393)

Plasmid

#250777

Purpose

Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

AraC with an AsLOV2 insertion behind S170 and C450A and K488N mutation

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Mutation

C450A, K488N

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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AraC-S170-LOV-C450A-D432V(M392)

Plasmid

#250776

Purpose

Expresses AraC-S170-LOV from a Trc promoter. For use in E. coli

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

AraC with an AsLOV2 insertion behind S170 and C450A and D432V mutation

Use

Synthetic Biology

Tags

His-tag

Expression

Bacterial

Mutation

C450A, D432V

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD-Flag WT

Plasmid

#249726

Purpose

Express GSDMD WT in mammalian cells

Depositor

Hao Wu

Article

Du et al Nature. 2024 Jun;630(8016):437-446. doi:

Insert

gasdermin D (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pJC2746 - pLC-U6-spacer-modified-tracer-EFS-eBFP2-P2A-Puro

Plasmid

#250277

Purpose

Empty backbone for cloning and stable sgRNA expression.

Depositor

Jacob Corn

Article

Muhar et al Nature. 2025 Feb;638(8050):519-527. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCBS-5737

Plasmid

#249326

Purpose

Expresses Non-targeting sgRNA

Depositor

Chase Beisel

Article

Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038

Insert

nontargeting sgRNA

Expression

Yeast

Mutation

N/A

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pTCB-pembroHCLALAPGknobHA

Plasmid

#237379

Purpose

pTwist CMV BetaGlobin encoding pembrolizumab heavy chain with LALA P329G knob Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

pembrolizumab heavy chain (silent Fc)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-pembroLC

Plasmid

#237380

Purpose

pTwist CMV BetaGlobin encoding pembrolizumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

pembrolizumab light chain

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-trasLC

Plasmid

#237365

Purpose

pTwist CMV BetaGlobin encoding trastuzumab light chain

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

trastuzumab light chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-rituxHCknob6xHis

Plasmid

#237371

Purpose

pTwist CMV BetaGlobin encoding rituximab heavy chain with knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

rituximab heavy chain

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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