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Lyn-iTrkA-mCh-CRY2

Plasmid

#106169

Purpose

mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

Lyn iTrkA mCherry CRY2PHR (CRY2 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLV(shRNA)-Puro-U6-CCEPR

Plasmid

#108277

Purpose

shRNA for CCEPR lncRNA

Depositor

Karl Munger

Article

Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

Insert

CCEPR (CCEPR Human)

Use

Lentiviral

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 S245X

Plasmid

#92366

Purpose

expression of M1 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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Flrt3-AP-His

Plasmid

#71949

Purpose

Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Flrt3 (Flrt3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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gp64-mVEGF-C-HisTag-pENTR

Plasmid

#64065

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (1-73) (72R)

Plasmid

#62082

Purpose

Expression of human p53 (1-73) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72R

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
pET28-p53 (1-73) (72P)

Plasmid

#62083

Purpose

Expression of human p53 (1-73) (72P) in e. coli

Depositor

Gary Daughdrill

Article

Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

Insert

p53 (TP53 Human)

Tags

HIs

Expression

Bacterial

Mutation

contains TAD residues 1-73; 72P

Promoter

T7

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
Human HMG-CoA lyase like 1 (HMGCLL1)

Plasmid

#52434

Purpose

mammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)

Depositor

Henry Miziorko

Article

Montgomery et al J Biol Chem. 2012 Sep 28;287(40):33227-3

Insert

HMGCLL1 (HMGCLL1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pX62

Plasmid

#46854

Purpose

TOP Hupki-puro2A-G245S plasmid to introduce mutant p53 to PLF ESC or MEFs by IMCE

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB-Puro-2A and Hupki p53 G245S (TP53 Mouse, Human)

Expression

Mammalian

Mutation

G245S

Available Since

Aug. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEYFPN3/GgVcl 1-1066 (YFP at C term)

Plasmid

#46278

Depositor

Susan Craig

Article

Chen et al J Cell Biol. 2005 May 9;169(3):459-70.

Insert

Vcl 1-1066 (VCL Chicken)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Cux2 in situ probe

Plasmid

#45623

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Cux2 in situ probe (Cux2 Mouse)

Use

In situ

Mutation

fragment contains nt 1069-1694 on NM_007804

Available Since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pENTR-MXC

Plasmid

#37140

Depositor

Robert Duronio

Article

White et al J Cell Biol. 2011 May 16;193(4):677-94.

Insert

Multi Sex combs (mxc Fly)

Use

Gateway Cloning

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA4.1/HisMax-Syx1

Plasmid

#35708

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx1 (Plekhg5 Mouse)

Tags

6x HIS and Xpress

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
yap1_R (OZ590)

Plasmid

#35230

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting yap1 (yap1 Zebrafish)

Use

Zebrafish targeting

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
yap1_L (OZ589)

Plasmid

#35229

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting yap1 (yap1 Zebrafish)

Use

Zebrafish targeting

Available Since

March 5, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-PAK2 S20A

Plasmid

#31672

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 20 to Alanine

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
p6596 MSCV-IP-N-HA ADRB2

Plasmid

#34895

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

ADRB2 (ADRB2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only