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Cdc2-DN-HA

Plasmid

#1889

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdc2 (CDK1 Human)

Tags

HA

Expression

Mammalian

Mutation

D146N, dominant negative

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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Halo-ATG16L1 HRD

Plasmid

#207537

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_psychLight1

Plasmid

#198739

Purpose

Expresses psychLight1 in mammalian cells

Depositor

Lin Tian

Article

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pX165-LZ3 Cas9

Plasmid

#140561

Purpose

Expresses LZ3 Cas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

N690C T769I G915M N980K

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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Halo-ATG9A HRD

Plasmid

#203222

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG9 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5B

Plasmid

#171947

Purpose

Expresses INPP5B in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5B (INPP5B Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-BCL9

Plasmid

#113759

Purpose

For the expression of FLAG-BCL9 in mammalian cells

Depositor

Mariann Bienz

Article

de la Roche et al BMC Cancer. 2008 Jul 15;8:199. doi: 10.1

Insert

BCL9 (BCL9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-EFS-dCas9-GFP

Plasmid

#64104

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

EFS

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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PB-Ostir-puro

Plasmid

#161972

Purpose

Expresses Ostir and puromycin resistance gene in mammalian cells

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Ostir-3xHA-p2a-puroR

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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560HTR

Plasmid

#24444

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis

Expression

Bacterial

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
pMRXIP SECFP-Stx17TM

Plasmid

#86777

Purpose

Expresses transmembrane domains of syntaxin17 tagged with SECFP in mammalian cells

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

transmembrane domains of human Syntaxin 17 (STX17 Human)

Use

Retroviral

Tags

SECFP

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pVTU_260_BirA

Plasmid

#63682

Purpose

Encodes E. coli biotin ligase gene (BirA) for expression in yeast cells

Depositor

Aaron Hoskins

Article

Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

Insert

BirA

Tags

6X Histidine and TEV cleavage site

Expression

Yeast

Promoter

ADH1/yeast

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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FUW-KO

Plasmid

#20331

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Klf4-F2A-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCAG-i53-bpA-EF1BFP

Plasmid

#111145

Purpose

Expression of i53 and a BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

i53 (mutL Synthetic)
BFP (BFP Synthetic)

Expression

Mammalian

Mutation

human Ubiquitin, contains 8 point mutations

Promoter

CAG promoter and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-SWAP70

Plasmid

#84581

Purpose

Expresses GFP-tagged SWAP70 in mammalian cells

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 (SWAP70 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-PHLPP1 N-terminal truncation

Plasmid

#22404

Purpose

Expresses a HA tagged, N-terminal truncation of human PHLPP1 in mammalian cells

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1 (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-3xFLAG-NCOA4

Plasmid

#192807

Purpose

Stable expression of 3xFLAG-NCOA4 in mammalian cells

Depositor

Noboru Mizushima

Article

Ohshima et al J Cell Biol. 2022 Oct 3;221(10). pii: 21

Insert

NCOA4 (NCOA4 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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peGFP-N1-SHB

Plasmid

#139605

Purpose

Expresses eGFP-SHB fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

SHB (SHB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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