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pCMV10/3xflag-Clock

Plasmid

#47334

Purpose

full length clock in pCMV10 with 3x flag at the N-terminus

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

clock

Tags

3x Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-enAsCas12f

Plasmid

#204642

Purpose

Expression of enAsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

enAsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D196K, N199K, G276R, N328G, D364R

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET47b-T7-enAsCas12f

Plasmid

#204642

Purpose

Expression of enAsCas12f in E. coli

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

enAsCas12f

Tags

6xHis

Expression

Bacterial

Mutation

D196K, N199K, G276R, N328G, D364R

Promoter

T7

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti GFP-AMOT p80

Plasmid

#32830

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p80 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only
Lenti GFP-AMOT p80

Plasmid

#32830

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p80 (AMOT Human)

Use

Lentiviral

Tags

GFP-AMOT

Expression

Mammalian

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only
4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps53-V5-6His

Plasmid

#175097

Purpose

V5 tagged Vps53 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps53 (Vps53 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEF6 Vps53-V5-6His

Plasmid

#175097

Purpose

V5 tagged Vps53 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

Vps53 (Vps53 Mouse)

Tags

V5

Expression

Mammalian

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-peroxi

Plasmid

#137806

Purpose

Visualization of peroxisomes

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

SKL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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VPS35 shRNA2

Plasmid

#163859

Purpose

shRNA targeting the coding sequences of VPS35

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

VPS35 retromer complex component (VPS35 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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VPS35 shRNA2

Plasmid

#163859

Purpose

shRNA targeting the coding sequences of VPS35

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

VPS35 retromer complex component (VPS35 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAG1160 mCherry-FIREtag

Plasmid

#205164

Purpose

Expresses mCherry -FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAG1160 mCherry-FIREtag

Plasmid

#205164

Purpose

Expresses mCherry -FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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YSBE 610 : pHYG_6xHA

Plasmid

#92091

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

6xHA

Use

tagging proteins in cryptococcus neoforman

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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