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Anti-VGlut3 [N34/34.2R]

Plasmid

#114494

Purpose

Mammalian Expression Plasmid of anti-VGlut3 (Rat). Derived from hybridoma N34/34.2.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-VGlut3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Slc17a8 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTFG011_Spike_Parental_D614G_3xFlag

Plasmid

#191571

Purpose

SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G mutation. Last 19 amino acids truncated.

Promoter

CMV

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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mRFP-Ub

Plasmid

#11935

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin (RBX1 Human)

Tags

mRFP

Expression

Mammalian

Available Since

July 5, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-PTRE-tight-hM3Dq-mCherry

Plasmid

#66795

Purpose

Expresses the DREADD receptor hM3Dq-mCherry under the control of tet-inducible promoter for use in TetTaging

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

pTIGHT promoter-hM3Dq-mCherry

Use

AAV

Expression

Mammalian

Promoter

pTIGHT TRE

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-dsRed-shvgat

Plasmid

#67845

Purpose

Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

Depositor

William Wisden

Article

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

h-synapsin

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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PKMC356_pTRE-ActivinA_IRES_BFP_WPRE

Plasmid

#225355

Purpose

Lentiviral vector for ActivinA and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Actvin A and TagBFP (Inhba Synthetic, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Nrp1-Fc-His

Plasmid

#72097

Purpose

Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp1 (Nrp1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

Plasmid

#190790

Purpose

[E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Inserts

Cas9
mazF-LVA

Use

CRISPR and Synthetic Biology

Tags

LVA

Expression

Bacterial

Mutation

silent mutations of BpiI-sites in original Cas9 g…

Promoter

PnrsB and Ptrc-[E*](104)

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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