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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 16,226 results for: Coli

Showing: 881 - 900 of 16226 results

  8. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  10. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  11. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  12. pHL1746

    Plasmid
    #53034
    Purpose
    PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ycdTlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. Ec ssb-pET(3d)

    Plasmid
    #237240
    Purpose
    over-express E. coli SSB
    Depositor
    Michael O'Donnell
    Insert
    ssb (SSB) (ssb E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    237240-plasmid-map-sequence-id-472868

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  14. pAIP4_1U8T

    Plasmid
    #234211
    Purpose
    Heterologous protein expression of flagellar motor switch protein FliM in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1U8T (fliM Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234211-plasmid-map-sequence-id-464176

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  15. pAIP4_1EW4

    Plasmid
    #234180
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EW4 (cyaY Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234180-plasmid-map-sequence-id-464145

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  16. pAIP4_3A7L

    Plasmid
    #234182
    Purpose
    Heterologous protein expression of glycine cleavage system H protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3A7L (GCV3 Escherichia coli K-12)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234182-plasmid-map-sequence-id-464147

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  17. pAIP4_3EYE

    Plasmid
    #234176
    Purpose
    Heterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3EYE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234176-plasmid-map-sequence-id-464141

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  18. pCASCADE-gapAP1

    Plasmid
    #87146
    Purpose
    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87146-plasmid-map-sequence-id-174938

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  19. pJK639

    Plasmid
    #72398
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72398-plasmid-map-sequence-id-133991

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Showing: 881 - 900 of 16226 results