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Showing: 881 - 900 of 1045 results

  6. lentiCRISPRv2-sgRNAs-del-AK057321-SVA-mcherry

    Plasmid
    #202821
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the SVA within the SVA-lncRNA AK057321 gene (exon 3) with EF-1apha for Cas9 and mcherry expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within SVA-lncRNA AK057321 gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (two)
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202821-plasmid-map-sequence-id-399746

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  7. pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR

    Plasmid
    #202830
    Purpose
    pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    CHAF1B intronic SVA del-VNTR sequence
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    202830-plasmid-map-sequence-id-399708

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  8. lentiCRISPRv2-sgRNAs-del-CHAF1B-SVA-BFP

    Plasmid
    #202825
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CHAF1B gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete intronic SVA within CHAF1B gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 - two
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202825-plasmid-map-sequence-id-399645

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  9. lentiCRISPRv2-sgRNAs-del-CDK5RAP2-SVA-BFP

    Plasmid
    #202824
    Purpose
    Lentiviral vector modified to express two sgRNAs that delete the intronic SVA within the CDK5RAP2 gene with EF-1apha for Cas9 and BFP expression
    Depositor
    Matthew Anderson
    Article
    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:
    Insert
    two sgRNAs (each with a U6 promoter) to delete SVA within CDK5RAP2 gene
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 - two
    Available since
    July 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202824-plasmid-map-sequence-id-399641

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  10. pUt-mNF-GFP

    Plasmid
    #199220
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199220-plasmid-map-sequence-id-396466

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  14. pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92319
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70a
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    92319-plasmid-map-sequence-id-182040

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  15. pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500

    Plasmid
    #92318
    Purpose
    Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    Optimized NTerm 6xHis tagged monomeric RFP (mRFP)
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    p70a
    Available since
    July 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    92318-plasmid-map-sequence-id-182037

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  16. pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500

    Plasmid
    #92230
    Purpose
    Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    NTerm 6xHis Tagged zmADH
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue V86 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92230-plasmid-map-sequence-id-181882

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  20. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid
    #196196
    Purpose
    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus
    Depositor
    Jürgen Knoblich
    Article
    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75
    Insert
    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    Use
    Homology repair donor
    Tags
    V5-APEX2-mScarlet-I
    Expression
    Mutation
    Promoter
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    196196-plasmid-map-sequence-id-385065

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Showing: 881 - 900 of 1045 results