We narrowed to 8,469 results for: PAN

1. pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212962

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_non-targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212963

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_GFP targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

   Plasmid

   #167583

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM5188

   Plasmid

   #120087

   Purpose

   Cyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.

   Depositor

   Susan Golden

   Article

   Chen et al Microbiology. 2016 Dec;162(12):2029-2041

   Insert

   pAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. NgOE FUNgGW

   Plasmid

   #92231

   Purpose

   Lentiviral expression of human Ng

   Depositor

   Weifeng Xu

   Article

   Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

   Insert

   Ng (NRGN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 011LP-pAAV-TNT4-EmGFP-pA

   Plasmid

   #120280

   Purpose

   AAV vector for cardiomyocyte specific expression of Emerald Green Fluorescent Protein using cardiac troponin T promoter

   Depositor

   Xander Wehrens

   Article

   Ni et al Circ Res. 2019 Jan 18;124(2):256-262. do

   Insert

   EmGFP
   

   Use

   AAV

   Tags

   3XFlag and HA

   Expression

   Mutation

   Promoter

   TNT Cardiac Troponin T promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

   Plasmid

   #202608

   Purpose

   Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-Kv
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. hKv2.1-HA

   Plasmid

   #111525

   Purpose

   Expresses human WT KCNB1 in mammalian cells

   Depositor

   Federico Sesti

   Article

   Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

   Insert

   human Kv2.1 + HA (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-DIO-GRAB_ATP1.0

   Plasmid

   #167581

   Purpose

   Express the ATP sensor GRAB_ATP1.0 in Cre positive cells

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP sensor GRAB_ATP1.0 (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hsyn-GRAB_ATP1.0mut

    Plasmid

    #167578

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in neurons

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

    Plasmid

    #202617

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    FRT-JEDI-1P-Kv
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCA15-Ubc-NLS- scFV-tdTomato

    Plasmid

    #199446

    Purpose

    Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

    Depositor

    Baohui Chen

    Article

    Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

    Insert

    scFV-tdTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C (UBC)

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

    Plasmid

    #202606

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

    Insert

    JEDI-1P-P2A-mCherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Neurod1-Atoh7HA3

    Plasmid

    #44438

    Depositor

    William Klein

    Article

    Mao et al Development. 2013 Feb;140(3):541-51. doi

    Insert

    Atoh7-3xHA knock-in (Atoh7 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Neurod1 left arm, Neurod1 right arm, and loxC2-Re…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcCas12nHS_RV6_PT1_empty

    Plasmid

    #203810

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6_PT1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ239A (dFnCas12a)-TV

    Plasmid

    #136379

    Purpose

    CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    dFnCas12a-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D917A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ141-ZmUbi-RZ-Aa3.8.4

    Plasmid

    #136375

    Purpose

    Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ292 (AaCas12b)-D570A-TV

    Plasmid

    #136380

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ290-D570A

    Plasmid

    #129673

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b-d570a entry …

    Tags

    Expression

    Plant

    Mutation

    D570A; AacCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ292-D570A-SRDX

    Plasmid

    #129684

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b-SRDX
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge