We narrowed to 5,822 results for: Pet2

1. pET29b-IPP1-His

   Plasmid

   #124137

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Inorganic pyrophosphatase (IPP1 Budding Yeast)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28C-mCherry-MED1LCD

   Plasmid

   #204404

   Purpose

   E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   MED1(947-1581) (MED1 Human)
   

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-Streptavidin-Glutamate_Tag

   Plasmid

   #46367

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

   Insert

   Streptavidin-Glutamate_Tag
   

   Tags

   6 glutamate tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET29b-AK1-His

   Plasmid

   #124135

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Adenylate kinase (AK1 Chicken)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-NmCas9-Flag

   Plasmid

   #109030

   Purpose

   Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-nanoHER2-SpyTag003

   Plasmid

   #216312

   Purpose

   Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   nanoHER2-SpyTag003
   

   Tags

   His6 tag and SpyTag003

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Tags

   C-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28 His6-mEGFP-D4

   Plasmid

   #226372

   Purpose

   Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4 (pfoA Clostridium perfringens)
   

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b-T7-6h-GST-α-Synuclein

   Plasmid

   #225224

   Purpose

   Alpha synuclein gene fused with gst gene under t7 promoter for bacterial expression of alpha synuclein protein.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   GST
   α-Synuclein
   

   Tags

   6xHis Tag and TEV Cleavage Site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-HIS-Tol2

    Plasmid

    #112486

    Purpose

    functional recombinant Tol2 protein from E.coli

    Depositor

    Stephen Ekker

    Article

    Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

    Insert

    Tol2 Transposase (tpase Synthetic)
    

    Tags

    HIS/6x

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-2H12.D11

    Plasmid

    #221145

    Purpose

    His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-tdTomato-CNA35

    Plasmid

    #61606

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    tdTomato and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28c-F30-Broccoli

    Plasmid

    #66788

    Purpose

    Expresses Broccoli in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Broccoli in the F30 scaffold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pet26-MBP-ELIC

    Plasmid

    #39239

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    Eriwinia chrysanthemi - pentameric ligand gated ion channel
    

    Tags

    His-10 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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