We narrowed to 901 results for: golden gate assembly
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Plasmid#219646PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest methodDepositorInsertALFA:HA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mGreenLantern:ALFA
Plasmid#219644PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest methodDepositorInsertmGreenLantern:ALFA SEED Cassette
Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-sfGFP:ALFA
Plasmid#219642PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest methodDepositorInsertsfGFP:ALFA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mGreenLantern
Plasmid#219643PurposePermits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest methodDepositorInsertmGreenLantern SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mCherry
Plasmid#219657PurposePermits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest methodDepositorInsertsfGFP SEED Cassette
Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-OLLAS:HA
Plasmid#219647PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest methodDepositorInsertOLLAS:HA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-Moontag:HA
Plasmid#219648PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest methodDepositorInsertMoonTag:HA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-EYFP:HA
Plasmid#219656PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest methodDepositorInsertEYFP:HA SEED Cassette
Available SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-mScarlet3:ALFA
Plasmid#219641PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest methodDepositorInsertmScarlet:ALFA SEED Cassette
Available SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKB-
Plasmid#62540PurposeCloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate CloningDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEED-LoxP-sfGFP:ALFA
Plasmid#219660PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.DepositorInsertsfGFP:ALFA SEED Cassette (LoxP)
Available SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX TALEN assembly system
Plasmid Kit#1000000063PurposeGolden Gate assembly of 15-16 RVD TALEN inserts in a single cloning step; designed to be used with high throughput roboticsDepositorAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ralstonia-GG-Kit
Plasmid Kit#1000000142PurposeBackbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniquesDepositorAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE Dicot Genome Editing Kit
Plasmid Kit#1000000084PurposeGolden Gate cloning-based assembly of constructs for CRISPR genome editing in dicotyledonous plants. Consists of "genome editing recipient" and "sgRNA shuttle" vectors.DepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Komagataeibacter Tool Kit (KTK)
Plasmid Kit#1000000216PurposeThe Komagataeibacter Tool Kit (KTK) is a standardized modular cloning system based on Golden Gate DNA assembly.DepositorAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
T2A Split/Link TurboID Kit
Plasmid Kit#1000000249PurposeThe T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.DepositorAvailable SinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
4G-Cloning Plasmid Kit
Plasmid Kit#1000000252PurposeThe 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.DepositorAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MoClo Plant Parts III: Transformation & Genome Engineering Kit
Plasmid Kit#1000000236PurposeThe MoClo Plant Parts III: Transformation & Genome Engineering Kit contains 94 Level 0 parts, compatible with the Phytobrick/MoClo system for golden gate assembly of plant transformation constructs.DepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
The Mammalian Toolkit
Plasmid Kit#1000000180PurposeA ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.DepositorAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only