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Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorInsertGα13-LgB106 (GNA13 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.H1-NP
Plasmid#134367PurposeNanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.DepositorInsertH1-NP (HRH1 Human)
UseTagsFlag and natural peptide of nanoluciferaseExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
MZB035
Plasmid#217834PurposeBacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplexDepositorUseTagsGSTExpressionBacterialMutationRemoved residues 618-1153 from AP3D1PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF
Plasmid#203549PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorUseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)
Plasmid#203548PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorUseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)
Plasmid#203547PurposeMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complexDepositorUseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-ATG13 (2-197)
Plasmid#188075PurposeTo make ATG101/ATG13 HORMA complex for expression in mammalian cellsDepositorInsertATG13 (ATG13 Human)
UseTagsstrep-strep-Flag tagExpressionMammalianMutationATG13 (2-197)PromoterCMVAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-INTS3-C
Plasmid#128642PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tagDepositorInsertINTS3 C-termini (INTS3 Human)
UseTagsHis tag on N and C-terminalExpressionBacterialMutationINTS3 C-termini (514–1042 aa)PromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
UQ4876
Bacterial Strain#37164DepositorBacterial ResistanceAmpicillin and kanamycinSpeciesBurkholderia xenovoransAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
MS2-P65-HSF1_GFP
Plasmid#61423PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.DepositorInsertMS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαs-LgB113
Plasmid#134362PurposeNanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.DepositorInsertGαs-LgB113 (GNAS Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA5-mVenus
Plasmid#185646PurposeNDUFA5 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA5 (NDUFA5 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFV2-mVenus
Plasmid#185653PurposeNDUFV2 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFV2 (NDUFV2 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFS6-mVenus
Plasmid#185806PurposeNDUFS6 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFS6 (NDUFS6 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA7-mVenus
Plasmid#185647PurposeNDUFA7 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA7 (NDUFA7 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
MZB053
Plasmid#217835PurposeBacterial expression of human AP3B1 (1-677) and AP3M1 AP-3 core hemicomplexDepositorUseTagsGSTExpressionBacterialMutationRemoved residues 678-1094 from AP3B1PromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
MBP-ATG13 (450-517)-ULK1 (836-1050)-His
Plasmid#222825PurposeBacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6HisDepositorUseTags6His and MBPExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBP-ATG13 (363-517)-ULK1 (836-1050)-His
Plasmid#222824PurposeBacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6HisDepositorUseTags6His and MBPExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only