We narrowed to 3,310 results for: multiplex

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nCas9-SpRY_P2A-mCherry

Plasmid

#219935

Purpose

Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-SpRY_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x2

Plasmid

#58772

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMSP3545-dCas9Str

Plasmid

#153516

Purpose

Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backbone

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

dCas9Str

Expression

Bacterial

Promoter

nisA

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLV hUbC-dCas9 VP64-T2A-GFP

Plasmid

#53192

Purpose

Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 VP64 T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

D10A and H840A

Promoter

hUbC

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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IK331

Plasmid

#233653

Purpose

Dox-inducible blue light-inducible Cre-N; Cre-N229-pmag

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-N229-pmag

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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IK332

Plasmid

#233654

Purpose

Dox-inducible blue light-inducible Cre-C; Cre-230C-nmag

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Cre-230C-nmag

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pCF.111

Plasmid

#227187

Purpose

pORTMAGE-Ec1 + supplement E. coli SSB

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

CspRecT, mutL_E32K, E. coli SSB

Expression

Bacterial

Mutation

mutL E32K

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pGLuc

Plasmid

#135288

Purpose

GBPart - GLuc Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Gaussia Luciferase

Use

Luciferase and Synthetic Biology

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131B

Plasmid

#69281

Purpose

Golden Gate entry vector; 1st gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163076

Purpose

Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pRU55

Plasmid

#167684

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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CT438

Plasmid

#233666

Purpose

Red light-inducible Flp-N; Flp-N27-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Flp-N27-PhyA

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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CT447

Plasmid

#233663

Purpose

Red light-inducible Flp-C; Flp-28C-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Flp-28C-FHY1

Expression

Mammalian

Mutation

None

Promoter

CAG

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRGE32

Plasmid

#63159

Purpose

Express sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplast

Depositor

Yinong Yang

Article

Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Rice snoRNA U3 promoter for gRNA/PTG expression a…

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxNeon

Plasmid

#229947

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CT769

Plasmid

#233661

Purpose

Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

PhyA-Flp-397-Flp-396-FHY1

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ133B

Plasmid

#69283

Purpose

Golden Gate entry vector; 3rd gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ132A

Plasmid

#69274

Purpose

Golden Gate entry vector; 2nd gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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