We narrowed to 15,354 results for: nts

1. pAGK43

   Plasmid

   #222223

   Purpose

   Edits NPAS2 Gene.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-28a(+)-His(6)-AtPCO1(wt)

   Plasmid

   #191798

   Purpose

   Full length wt AtPCO1 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO1
   

   Tags

   6xHis, Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a(+)-His(6)-AtPCO2(wt)

   Plasmid

   #191799

   Purpose

   Full length wt AtPCO2 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO2
   

   Tags

   6xHis, Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a(+)-His(6)-AtPCO3(wt)

   Plasmid

   #191800

   Purpose

   Full length wt AtPCO3 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO3
   

   Tags

   6xHis, Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLZRS-panTRK-CR1-EGFP

   Plasmid

   #187194

   Purpose

   To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

   Depositor

   Pantelis Tsoulfas

   Article

   O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

   Insert

   CR1 pan-TRK receptor
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   mo-MLV or MMLV

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMYA5/pBSSK+

   Plasmid

   #97205

   Purpose

   CMYA5 (NM_153610, nt 12205 to 12279) in pBSSK+

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   CMYA5 (CMYA5 Human)
   

   Use

   Unspecified

   Available Since

   July 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. CMYA5/pENTR3C

   Plasmid

   #97408

   Purpose

   CMYA5 (NM_153610, nt 12205 to 12279) in pENTR3C, Gateway Entry vector

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   CMYA5 (CMYA5 Human)
   

   Use

   Unspecified

   Available Since

   July 18, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCRII-Topo Tmtc4

   Plasmid

   #45869

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   TMTC4 in situ probe (Tmtc4 Mouse)
   

   Use

   In situ

   Mutation

   nt 1220-1798 on BC031368

   Available Since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

9. MDH1-pre-let-7_PShairpin

   Plasmid

   #26537

   Depositor

   Chang-Zheng Chen

   Article

   Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

   Insert

   pre-let-7_PS hairpin
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   5 nt mutated to make pre-let-7 stem pairing perfe…

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmCherry-MS2coatProtein

    Plasmid

    #103832

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mCherry-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TL1 DdCBE T2 (DddA6-N)

    Plasmid

    #221993

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: Q1310R, S1330I, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. 7i sgRNA for 4-μHOM reporter

    Plasmid

    #113626

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7i sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-dSa-VPR mini.-1X snRP1

    Plasmid

    #99687

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNL4-3 envFS eGFP Δvpu/env

    Plasmid

    #101345

    Purpose

    Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta vpu/Env

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21c(+)-mtLeuRS

    Plasmid

    #31079

    Depositor

    Linda Spremulli

    Article

    Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

    Insert

    Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Includes coding sequence for the mature form. Nt …

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

    Plasmid

    #205268

    Purpose

    Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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