We narrowed to 5,282 results for: pcdna3.1

1. pcDNA3.1(+)-FluoSTEP-AktAR

   Plasmid

   #181856

   Purpose

   Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-AktAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MS2-HA-HsNot4_AA

   Plasmid

   #148255

   Purpose

   Mammalian Expression of HsNot4

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   HsNot4 (CNOT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation compared to the sequence give…

   Promoter

   Available since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hFluc-V5-6xbsMS2_U

   Plasmid

   #147639

   Purpose

   Mammalian Expression of 6xbsMS2

   Depositor

   Elisa Izaurralde

   Insert

   6xbsMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) Drp1 V2 S600D

   Plasmid

   #46345

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S600D

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+) Drp1 V2 S600A

   Plasmid

   #46344

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S600A

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pY129 (pcDNA3.1-U6-huMb3Cpf1)

   Plasmid

   #92305

   Purpose

   Expression of humanized Mb3Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+) HIPK3 Sense

   Plasmid

   #60634

   Purpose

   Expresses the human HIPK3 exon 2 circular RNA

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

   Insert

   Homeodomain-interacting protein kinase 3 (HIPK3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1+_V5-ANKRD52

   Plasmid

   #92012

   Purpose

   Expresses V5-ANKRD52

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   ANKRD52 (ANKRD52 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-Golgi-RasAR

   Plasmid

   #205570

   Purpose

   Golgi membrane-targeted RasAR.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   Golgi-RasAR (RAF1, GOLGB1 Synthetic, Human)
   

   Use

   Tags

   Golgin B1 (Giantin) residues 3131-3259

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pY018 (pcDNA3.1-hPdCpf1)

    Plasmid

    #69990

    Purpose

    Expresses humanized PdCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPdCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1 hSCN5A-L325R

    Plasmid

    #145373

    Purpose

    expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells

    Depositor

    Hugues Abriel

    Article

    Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9.

    Insert

    sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 325 to Arginine

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid

    #92303

    Purpose

    Expression of humanized TsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1/V5-His-TN-DROSHA

    Plasmid

    #106252

    Purpose

    Express TN-DROSHA in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Chang et al Genome Res. 2015 Sep;25(9):1401-9. doi:

    Insert

    Drosha Ribonuclease III (DROSHA Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    E1045Q and E1222Q mutations

    Promoter

    CMV

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-RABIF-FLAG

    Plasmid

    #135558

    Purpose

    Express human RABIF in mammalian cells, FLAG at N terminal

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid

    #126472

    Purpose

    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    Or47a (Or47a Fly)
    

    Use

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MS2-HA-HsUNR_AA

    Plasmid

    #148239

    Purpose

    Mammalian Expression of HsUNR

    Depositor

    Elisa Izaurralde

    Insert

    HsUNR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 EYFP H148Q

    Plasmid

    #25871

    Depositor

    Peter Haggie

    Article

    Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

    Insert

    EYFP H148Q
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP from pEYFP (Clontech) with Histidine 148 to …

    Promoter

    Available since

    Aug. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-MS2-HA-HsMex3B_AD

    Plasmid

    #148478

    Purpose

    Mammalian Expression of HsMex3B

    Depositor

    Elisa Izaurralde

    Insert

    HsMex3B (MEX3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-HA-BLMP-1-pcDNA3.1-

    Plasmid

    #52512

    Purpose

    expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Blmp-1 (blmp-1 Nematode)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    N75S mutation compared GenBank reference sequence…

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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