We narrowed to 166,303 results for: addgene

1. pAAVS1-Neo-TNNT2-Blast

   Plasmid

   #214014

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-Neo-TNNT2-Hygro

   Plasmid

   #214015

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAVS1-Blast-TNNT2-Zeo

   Plasmid

   #214018

   Purpose

   Expression of cardiac troponin T

   Depositor

   Paul Burridge

   Article

   Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

   Insert

   TNNT2 (TNNT2 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Parkin(C431N)-A92mKO2

   Plasmid

   #213545

   Purpose

   Transient mammalian expresssion of ligase-dead C431N Parkin, internally tagged with mKO2

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Expression

   Mammalian

   Mutation

   C431N mutation in Parkin

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLv-EF1a-Parkin(R104A)-A92mKO2

   Plasmid

   #213556

   Purpose

   Lentiviral vector for stable expression of R104A Parkin, internally tagged with mKO2

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   R104A mutation in Parkin

   Promoter

   EF1a

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLv-CMV(trunc)-Parkin-A92mKO2

   Plasmid

   #213549

   Purpose

   Lentiviral vector for stable low expression of Parkin, internally tagged with mKO2

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Truncated CMV

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLv-EF1a-Parkin(N273K)-A92mKO2

   Plasmid

   #213555

   Purpose

   Lentiviral vector for stable expression of N273K Parkin, internally tagged with mKO2

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N273K mutation in Parkin

   Promoter

   EF1a

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLv-EF1a-Parkin(W403A)-A92mKO2

   Plasmid

   #213558

   Purpose

   Lentiviral vector for stable expression of W403A Parkin, internally tagged with mKO2

   Depositor

   Steven Altschuler, Lani Wu

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   W403A mutation in Parkin

   Promoter

   EF1a

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Solo-sTagRFP-TUBA1B

   Plasmid

   #207765

   Purpose

   Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

    Plasmid

    #174895

    Purpose

    amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    102TAG 150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

    Plasmid

    #190876

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFPnls-barcode210
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-EGFP barcode-9-SV40 polyA

    Plasmid

    #190873

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode9
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEos3.2trLAT-P

    Plasmid

    #203747

    Purpose

    Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLAToC27A (Lat Mouse)
    

    Tags

    ER translocation signal - mEos3.2

    Expression

    Mammalian

    Mutation

    C27A

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. trLATm1PtoLmEos3.2

    Plasmid

    #203749

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Prolines P8 and P17 in the LAT transmembrane domain mutated to L. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm1 (PtoL) (LAT Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Mutation

    P8L, P17L

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

    Plasmid

    #197424

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

    Plasmid

    #197425

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3

    Plasmid

    #197426

    Purpose

    π-element knock-in into exon 5 of MAPRE1

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE1 gRNA #3 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
    

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LENTICRISPR-SNRPA_sgRNA1

    Plasmid

    #201622

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    SNRPA (SNRPA Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LENTICRISPR-SNRPA_sgRNA2

    Plasmid

    #201623

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    SNRPA (SNRPA Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LENTICRISPR-NDUFB5_sgRNA1

    Plasmid

    #201606

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    NDUFB5 (NDUFB5 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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