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pET-22HT-MSI1(7-192)R53E/R59E/R61E

Plasmid

#60355

Purpose

R53E R59E R61E triple mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E, R59E and R61E mutations; contains amino aci…

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA TMEM 138-flag

Plasmid

#41628

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 138 (TMEM138 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-bpNLS-eeBxb1 (CJT555)

Plasmid

#248209

Purpose

eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-eeBxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

eeBxb1(V74A, E229K, V375I)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1-T2A-Cre

Plasmid

#189011

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pVB240306

Plasmid

#250660

Purpose

E. coli expression of the GSM domain of mouse ChREBPalpha (aa 43-207)

Depositor

Charles Brenner

Article

Tiwari et al Nat Metab. 2025 Nov;7(11):2284-2299. doi

Insert

ChREBP alpha GSM domain (Mlxipl Mouse)

Tags

6xHis

Expression

Bacterial

Mutation

contains aa 43-307 only, with optimized E. coli c…

Promoter

T7

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-Intergenic sgRNA

Plasmid

#237463

Purpose

All-in-one base editor expressing adenine base editor (ABE8e-nSpCas9) and gRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-Intergenic sgRNA

Plasmid

#237464

Purpose

All-in-one base editor expressing cytosine base editor (evoCDA1-nSpCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9-Intergenic sgRNA

Plasmid

#237465

Purpose

All-in-one base editor expressing adenine base editor (ABE8e-nSaCas9) and sgRNA control targeting Intergenic site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

Intergenic sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-ABE8e-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237466

Purpose

All-in-one adenine base editor (ABE8e-nSpCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX461-evoCDA1-nSpCas9-NRF1-ss-sgRNA

Plasmid

#237467

Purpose

All-in-one cytosine base editor (evoCDA1-nSpCas9) with sgRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

sgRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9-NRF1-ss-sgRNA

Plasmid

#237468

Purpose

All-in-one adenine base editor (ABE8e-nSaCas9) with gRNA targeting NRF1-Ex7 splice site

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

gRNA targeting NRF1-Ex7 splice site

Use

CRISPR

Expression

Mammalian

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-miniTurbo-NRF1-∆ex7

Plasmid

#237454

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of miniTurbo fused to NRF1 isoform lacking the alternative exon-7 (∆ex7)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 ∆exon-7

Tags

miniTurbo

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-NRF1-FL_WobbleMut

Plasmid

#237455

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full-length NRF1 isoform mutated to be resistant to NRF1 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 full length

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-NRF1-∆ex7_WobbleMut

Plasmid

#237456

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged NRF1 isoform lacking the alternative exon-7 (∆ex7) mutated to be resistant to NRF1 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

NRF1 ∆exon-7

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pCambia1300_cLUC-NLRa

Plasmid

#233526

Purpose

Expresses split luciferase fusion cLUC-NLRa in planta

Depositor

Brande Wulff

Article

Chen et al Science. 2025 Mar 28;387(6741):1402-1408

Insert

Sr62NLRa

Tags

C-terminal fragment of Luciferase

Expression

Plant

Promoter

35S

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEN243-KH217

Plasmid

#224550

Purpose

Cas9-2A-puro and sgRNA targeting the STOP codon mouse Car2

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

Cas9 and sgRNA targeting the STOP codon of mouse CAR2 (Car2 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-W174A-EGFP

Plasmid

#179933

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing W174A mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

Cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

W174A

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1α-F44E-EGFP

Plasmid

#179936

Purpose

C terminal fusion of EGFP to mouse HP1α (Cbx5) containing F44E mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx5 (Cbx5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

F44E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1α-I165E-EGFP

Plasmid

#179937

Purpose

C terminal fusion of GFP to mouse HP1α (Cbx5) containing I165E mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx5 (Cbx5 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I165E

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HP1γ-M158V-EGFP

Plasmid

#179927

Purpose

C terminal fusion of EGFP to mouse HP1γ (Cbx3) containing M158V mutation

Depositor

Andrew Newman

Article

Newman et al Nat Commun. 2025 Aug 8;16(1):7325. doi:

Insert

cbx 3 (Cbx3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

M158V

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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