We narrowed to 166,303 results for: addgene

1. LENTICRISPR-NDUFB5_sgRNA2

   Plasmid

   #201607

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   NDUFB5 (NDUFB5 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK mCh-V5-NES-EGFP-EGFR NLS zeo

   Plasmid

   #192337

   Purpose

   Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

   Plasmid

   #192339

   Purpose

   Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT mCh-V5-NES-EGFP-EGFR NLS

   Plasmid

   #192315

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TT mCh-V5-NES-EGFP-Erbb2 NLS

   Plasmid

   #192317

   Purpose

   Gateway entry vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p426-Cup1p-FusionRed

   Plasmid

   #188392

   Purpose

   Cu2+-dependent expression of red fluorescent protein in yeast cells

   Depositor

   Michihiko Kataoka

   Article

   Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

   Insert

   FusionRed
   

   Expression

   Yeast

   Promoter

   CUP1 promoter

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GLRX-roGFP2 in pT3TS-Dest

   Plasmid

   #194297

   Purpose

   In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK

   Depositor

   Rob Parton

   Article

   Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

   Insert

   GLRX-roGFP2
   

   Use

   In vitro transcription of mrna

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCALNL-tdTomato-2A-mSyp::EGFP

   Plasmid

   #191213

   Purpose

   Expresses tdTomato protein and mouse Synaptophysin protein fused with EGFP in Cre expressing mammalian cells.

   Depositor

   Theofanis Karayannis

   Article

   Gesuita et al STAR Protoc. 2022 Nov 11;3(4):101837. do

   Insert

   tdTomato-T2A-mSyp::EGFP (Syp Mouse)
   

   Use

   Pcagen

   Tags

   mSyp::EGFP

   Expression

   Mammalian

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-EGFP barcode-7-SV40 polyA

   Plasmid

   #190871

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode7
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-EGFP barcode-8-SV40 polyA

    Plasmid

    #190872

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode8
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-EGFP barcode-6-SV40 polyA

    Plasmid

    #190870

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode6
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCL.179

    Plasmid

    #185000

    Purpose

    Express -Eco1 HEK4 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    HEK4 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-double floxed-Gat1(R69K)-HA-WPRE-HGHpA

    Plasmid

    #184636

    Purpose

    Expresses the GABA membrane transporter Gat1 R69K mutant fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Mutation

    Gat1 gene with Arginine 69 changed to Lysine, to …

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK880-pCAG-DmrC-NLS-3xFLAG-VP64

    Plasmid

    #136912

    Purpose

    DmrC with VP64 fused to it's C-terminus; Mammalian expression vector

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

    Insert

    pCAG-DmrC-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Chicken Beta Actin

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rcan1_pLENTI-CAG-IRES-GFP

    Plasmid

    #177000

    Purpose

    Mammalian lentiviral expression vector encoding Rcan1

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    Rcan1 (Rcan1 Mouse)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. RWDD2B_pLENTI-CAG-IRES-GFP

    Plasmid

    #177001

    Purpose

    Mammalian lentiviral expression vector encoding RWDD2B

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    RWDD2B (RWDD2B Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV Tmx4-V5 C209A

    Plasmid

    #175140

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C209A

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV Tmx4-V5 C211A

    Plasmid

    #175139

    Purpose

    Lentiviral expression of V5-tagged mouse Tmx4

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Tmx4 (Tmx4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    C211A

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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