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pDONR223_CTNNB1_p.G34E

Plasmid

#81727

Purpose

Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

G34E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.G34R

Plasmid

#81697

Purpose

Gateway Donor vector containing CTNNB1,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

G34R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR D-TOPO-C3_9

Plasmid

#60258

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1S1_WT

Plasmid

#82091

Purpose

Gateway Donor vector containing AKT1S1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1S1 (AKT1S1 Human)

Use

Gateway entry vector

Mutation

A47P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DPY30_WT

Plasmid

#82109

Purpose

Gateway Donor vector containing DPY30 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DPY30 (DPY30 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_MFSD5

Plasmid

#131941

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD5 (MFSD5 Human)

Expression

Mammalian

Available Since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO_NWS.AU1.VSVg_NGFR

Plasmid

#158239

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

NWS.AU1.VSVg

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC27A1_STOP

Plasmid

#161294

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A1 (SLC27A1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_MFSD11

Plasmid

#131928

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD11 (MFSD11 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_HTR4_WT

Plasmid

#81915

Purpose

Gateway Donor vector containing HTR4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HTR4 (HTR4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BTC_WT

Plasmid

#81904

Purpose

Gateway Donor vector containing BTC , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BTC (BTC Human)

Use

Gateway entry vector

Mutation

L124M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_WT

Plasmid

#82885

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A17

Plasmid

#131991

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A17 (SLC25A17 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_STK3_WT

Plasmid

#82319

Purpose

Gateway Donor vector containing STK3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK3 (STK3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DDIT4_WT

Plasmid

#82254

Purpose

Gateway Donor vector containing DDIT4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DDIT4 (DDIT4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CTNNB1_p.T257I

Plasmid

#81718

Purpose

Gateway Donor vector containing CTNNB1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CTNNB1 (CTNNB1 Human)

Use

Gateway entry vector

Mutation

T257I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BAP1_p.N299S

Plasmid

#81572

Purpose

Gateway Donor vector containing BAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BAP1 (BAP1 Human)

Use

Gateway entry vector

Mutation

N299S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BAP1_p.D75G

Plasmid

#81526

Purpose

Gateway Donor vector containing BAP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BAP1 (BAP1 Human)

Use

Gateway entry vector

Mutation

D75G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ACAA1_WT_V5

Plasmid

#82978

Purpose

Gateway Donor vector containing ACAA1 used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ACAA1 (ACAA1 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TSTA3_WT_V5

Plasmid

#82967

Purpose

Gateway Donor vector containing TSTA3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TSTA3 (TSTA3 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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