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pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA1.1

Plasmid

#64116

Purpose

Vector for expression of St1 sgRNA1.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA1.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMX1418

Plasmid

#127856

Purpose

Expresses EGFP-IPNN-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

IPNN-MCAK

Tags

EGFP

Expression

Mammalian

Mutation

mutation is I97N and P98N

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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7iBi89 (rat fibronectin)

Plasmid

#14065

Depositor

Richard Hynes

Article

Huh et al Mol Cell Biol. 1993 Sep . 13(9):5301-14.

Insert

Rat Fibronectin 7iBi89 (Fn1 Rat)

Expression

Mammalian

Mutation

Insert was cut out of pGEM3 with SalI and SacI an…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pX165-xCas9

Plasmid

#140568

Purpose

Expresses xCas9 in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

Cas9

Mutation

A262T R324L S409I E480K E543D M694I E1219V

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-INPP5J

Plasmid

#171949

Purpose

Expresses INPP5J in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5J (INPP5J Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.scr_2

Plasmid

#190704

Purpose

Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Scrambled sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAV0523

Plasmid

#133495

Purpose

Expression of nucleo-cytoplasmic sfGFP in M-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(mam1*)-sfGFP-terminator(ScADH1)-kanMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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p1.2-Zeo

Plasmid

#162740

Purpose

Protein expression in CHO cells

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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SH4-FRB-mRFP

Plasmid

#100741

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

Fyn

Tags

FRB and mRFP

Expression

Mammalian

Mutation

SH4 domain only

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAV0543

Plasmid

#133493

Purpose

Expression of nucleo-cytoplasmic mCherry in P-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Xl-VSP1-GFP

Plasmid

#51883

Purpose

expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pKIOct4-Halo-IRES-Bsd

Plasmid

#115689

Purpose

Knock-in of a HaloTag fusion protein into the endogenous OCT4 (Pou5f1) protein

Depositor

David Suter

Article

Strebinger et al Mol Syst Biol. 2019 Sep;15(9):e9002. doi

Insert

OCT4-Halo-IRES-Bsd

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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MSGV Rh Acceptor PGK-NGFR

Plasmid

#64272

Purpose

Retroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFR

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMX1433

Plasmid

#127857

Purpose

Expresses EGFP-deltaN-term-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

deltaN-term-MCAK

Tags

EGFP

Expression

Mammalian

Mutation

deletion of first 19 codons of MCAK

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB-GCN

Plasmid

#127476

Purpose

coding sequence for synthetic zinc finger ZF-BCB-GCN

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper

Use

Cell-free transcription–translation

Tags

GCN4 leucine zipper

Promoter

T7

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pOBCol3.6-MCS

Plasmid

#108311

Purpose

Drive inserted gene in type 1 collagen expressing cells.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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