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pSC-humanADAR1

Plasmid

#198235

Purpose

Expresses human ADAR1 in yeast

Depositor

Brenda Bass

Publication

Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

Insert

human ADAR1 (ADAR Human)

Tags

His10_TEV

Expression

Yeast

Promoter

GAL1

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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MYR-FR-C2D-EGFR

Plasmid

#179262

Purpose

Light-induced clustering of EGFR cytosolic domains

Depositor

Jared Toettcher

Publication

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Cry2 PHR, FUS, FusionRed, and Myristoylation sequ…

Promoter

SFFV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-YTHDC1-FL-mCherry-Cry2

Plasmid

#177124

Purpose

Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Publication

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YTHDC1 (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

SFFV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Publication

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV 4xSTAR-mScarletI-NLS-blast

Plasmid

#136260

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Publication

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 4 repeats

Use

Lentiviral

Tags

mScarletI-NLS

Expression

Mammalian

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2J1

Plasmid

#124669

Purpose

Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Publication

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2J1 (UBE2J1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/TagRFP-LL5b

Plasmid

#112828

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński, Joshua Sanes

Publication

Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LL5b (Phldb2 Mouse)

Tags

TagRFP

Expression

Mammalian

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p2K7-bsd-UBI-tagRFP-KDEL

Plasmid

#114179

Purpose

Lentiviral vector for expression of tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Publication

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Insert

tagRFP-KDEL

Use

Lentiviral

Tags

KDEL retention signal and signal peptide

Expression

Mammalian

Promoter

Ubiquitin

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458_Ruby

Plasmid

#110164

Purpose

Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

Depositor

Philip Hublitz

Publication

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9

Use

CRISPR

Tags

3xFLAG and Ruby2

Expression

Mammalian

Available since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCMV-Tag2-Sox9 T236A

Plasmid

#111455

Purpose

Transient expression of Flag-tagged human Sox9 with T236A mutation

Depositor

Sharon Pine

Publication

Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

Insert

Sox9 (SOX9 Human)

Tags

Flag

Expression

Mammalian

Mutation

T236A mutation

Promoter

CMV

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-Ag43

Plasmid

#107743

Purpose

Expresses Ag43 under the control of an L-arabinose transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Publication

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

Ag43 (flu )

Expression

Bacterial

Mutation

removed all pstI cut sites with silent mutations,…

Promoter

pBAD

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRIP-SFFV-mtagBFP-2A STING-342STOP

Plasmid

#102588

Purpose

Lentivector to express STING delta 342 and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta 342

Promoter

SFFV

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pENTR221 UBR5 ∆UBR

Plasmid

#81063

Purpose

Gateway entry vector encoding full length UBR5 W1235L (∆UBR)

Depositor

Darren Saunders

Publication

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

Ubiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)

Use

Cloning

Mutation

W1235L (bp g3704t)

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ef1a-mCh-Puro-GFPi

Plasmid

#31848

Depositor

Bruce Conklin

Publication

Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

Insert

GFP RNAi

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; Ef1alpha for mCherry-puromycin

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSIREN-RetroQ-CDK6-shRNA

Plasmid

#25789

Depositor

Judy Lieberman

Publication

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

shRNA against human CDK6 (CDK6 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCS2 hSmad1-GM

Plasmid

#22992

Depositor

Edward De Robertis

Publication

Fuentealba et al Cell. 2007 Nov 30. 131(5):980-93.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Smad1 (SMAD1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S210A ,T202A, S191A, S198A

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
TDP-43 WT GFP

Plasmid

#237542

Purpose

Expression of GFP-tag fused to wild-type TDP-43

Depositor

Tatyana Shelkovnikova

Publication

Matsukawa et al RNA Biol. 2021 Jan 11:1-9. doi: 10.1080/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP-tagged TDP-43 WT (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

wild-type TDP-43

Promoter

CMV

Available since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSC-humanADAR1

Plasmid

#198235

Purpose

Expresses human ADAR1 in yeast

Depositor

Brenda Bass

Publication

Eggington et al Nat Commun. 2011;2:319. doi: 10.1038/nco

Insert

human ADAR1 (ADAR Human)

Tags

His10_TEV

Expression

Yeast

Promoter

GAL1

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MYR-FR-C2D-EGFR

Plasmid

#179262

Purpose

Light-induced clustering of EGFR cytosolic domains

Depositor

Jared Toettcher

Publication

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Cry2 PHR, FUS, FusionRed, and Myristoylation sequ…

Promoter

SFFV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pHR-YTHDC1-FL-mCherry-Cry2

Plasmid

#177124

Purpose

Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Publication

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

YTHDC1 (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

SFFV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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