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SG-C-Int-8

Plasmid

#117037

Purpose

Constitutive integrase cassette - Tp901-Int5

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Tp901-Int5

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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SG-C-Int-9

Plasmid

#117038

Purpose

Constitutive integrase cassette - Tp901-Int7

Depositor

Jerome Bonnet

Article

Guiziou et al Nat Commun. 2019 Jan 28;10(1):456. doi:

Insert

Constitutive integrase cassette - Tp901-Int7

Use

Synthetic Biology

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAW8

Plasmid

#110222

Purpose

Cre-expression plasmid for cassette exchange

Depositor

Tony Carr

Article

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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p1wtVT-lacI

Plasmid

#107945

Purpose

Ratiometric reporter [PT7-RBSB0034, high copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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p4eng3VT-lacI

Plasmid

#107952

Purpose

Ratiometric reporter [PT7(-3G)-RBST7g10, low copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p1eng3VT-lacI

Plasmid

#107951

Purpose

Ratiometric reporter [PT7(-3G)-RBST7g10, high copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p1enVT-lacI

Plasmid

#107949

Purpose

Ratiometric reporter [PT7-RBST7g10, high copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p4g3VT-lacI

Plasmid

#107948

Purpose

Ratiometric reporter [PT7(-3G)-RBSB0034, low copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p1g3VT-lacI

Plasmid

#107947

Purpose

Ratiometric reporter [PT7(-3G)-RBSB0034, high copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTSara*

Plasmid

#107955

Purpose

Arabinose-inducible split T7*RNAP

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Mutation

R632S

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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p4wtVT-lacI

Plasmid

#107946

Purpose

Ratiometric reporter [PT7-RBSB0034, low copy number]

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

mVenus

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTSaraSZ

Plasmid

#107956

Purpose

Arabinose-inducible split T7RNAP with SynZIP domains 17/18

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

T7RNAP (modified)

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

Plasmid

#141391

Purpose

Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-N (N SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro

Plasmid

#141385

Purpose

Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-E (E SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

Plasmid

#141378

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

Plasmid

#141373

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

Plasmid

#141386

Purpose

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-M (M SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IRES-Puro

Plasmid

#141367

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp1 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge