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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,083 results for: nes

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Showing: 9101 - 9120 of 16083 results

  3. pQ-Elo-HB-C-A(1-772)

    Plasmid
    #171085
    Purpose
    Co-expresses of WT-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Inserts
    Elongin B (ELOB Human)
    Elongin C (ELOC Human)
    Elongin A (ELOA Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Deleted N-term 26 amino acids for expression purp…
    Promoter
    T5 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171085-plasmid-map-sequence-id-335501

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  4. p663-UBC-miniTurbo-V5-CDK11A_IDG-K

    Plasmid
    #214461
    Purpose
    Gateway destination clone of CDK11A (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDK11A (CDK11A Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214461-plasmid-map-sequence-id-423211

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  10. pLJM1-Flag-Rheb-4Rs

    Plasmid
    #165022
    Purpose
    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-4Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    change lysine residues on position 109, 135, 151,…
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165022-plasmid-map-sequence-id-326717

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  11. Mouse 3' Hp1a AID GFP PuroR

    Plasmid
    #127897
    Purpose
    HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene
    Depositor
    Mark Groudine
    Insert
    Hp1a (Cbx5 Mouse, Mustard Weed)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Mutation
    Inserting GFP AID 2A Puro into mouse 3' Hp1a
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127897-plasmid-map-sequence-id-250990

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  15. pTXB1-hMeCP2-G273X

    Plasmid
    #48093
    Purpose
    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X
    Depositor
    Huda Zoghbi
    Article
    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10
    Insert
    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    Expression
    Bacterial
    Mutation
    Mxe intein/chitin binding domain tagged, expresse…
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-srIκBα

    Plasmid
    #242411
    Purpose
    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (E3, NSs, L*) and inflammatory signaling inhibitor, super repressor inhibitor of κBα (srIκBα).
    Depositor
    Ewan St. John Smith
    Article
    Lim et al bioRxiv 2024.09.24.614636
    Inserts
    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-srIκBα
    Use
    Mouse Targeting; Self-amplifying rna
    Expression
    Mammalian
    Mutation
    serines 32 and 36 to alanines
    Available Since
    Sept. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    242411-plasmid-map-sequence-id-487192

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  20. pCKC764

    Plasmid
    #226625
    Purpose
    piggyBAC pCAG-hCas9-T2A-Unbiased3
    Depositor
    Chang Liu
    Article
    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.
    Inserts
    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT S. pyogenes, Chicken, Human)
    EF1a promoter, blasticidin resistance
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV and EF1a
    Available Since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    226625-plasmid-map-sequence-id-449160

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Showing: 9101 - 9120 of 16083 results