We narrowed to 12,131 results for: LEA

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CT336

Plasmid

#233662

Purpose

AND Gate Reporter to identify Cre and Flp recombination; FRT-STOP-FRT-loxP-STOP-loxP-GFP

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

FRT-STOP-FRT-loxP-STOP-loxP-GFP

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pISEre1reRNA

Plasmid

#226821

Purpose

Expressing ISEre1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WW+

Plasmid

#231885

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WW+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

T7

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 K5

Plasmid

#231866

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 K5

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 K5

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103K, D115K, D127K, D133K, E143K

Promoter

T7

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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Cdk13_intron4_sg4_pX330

Plasmid

#127341

Purpose

sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Mouse Cdk13 Intron 4 Targeting sgRNA

Use

CRISPR and Mouse Targeting

Promoter

U6 Promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-C-GFP-NLS

Plasmid

#222604

Purpose

Expression of MAC3-tagged GFP-NLS (C-terminal)

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

EGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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Tol2-U6.3-sgRNA-empty-GFP

Plasmid

#221843

Purpose

empty vector to clone custom sgRNA into BsaI sites to express sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoter

Depositor

Stephen Terry

Article

O'Sullivan et al Development. 2024 Aug 1;151(15):dev20284

Insert

EGFP

Use

CRISPR; Tol2 transposon optimised for chick expre…

Expression

Mammalian

Promoter

CAGC

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pSAVED-CHAT_PCaspase-N-term

Plasmid

#214044

Purpose

Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

SAVED-CHAT-PCaspase

Expression

Bacterial

Mutation

aa 1-153

Promoter

lacUV5 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1318

Plasmid

#217960

Purpose

Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

L63A, F66A, I71A

Promoter

CMV

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M1_YLVV

Plasmid

#197081

Purpose

This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M1, which contains the YLVV signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M2_YVPM

Plasmid

#197082

Purpose

This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M2, which contains the YVPM signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M3_YENL

Plasmid

#197083

Purpose

This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M3, which contains the YENL signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M5_YESP

Plasmid

#197085

Purpose

This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M5, which contains the YESP signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M6_ITYAAV

Plasmid

#197086

Purpose

This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M6, which contains the ITYAAV signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M7_PVQP

Plasmid

#197087

Purpose

This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M7, which contains the PVQP signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M8_RSTKP

Plasmid

#197088

Purpose

This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M8, which contains the RSTKP signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMAT_M9_PVQE

Plasmid

#197091

Purpose

This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP

Depositor

Wendell Lim

Article

Daniels et al Science. 2022 Dec 16;378(6625):1194-1200

Insert

This plasmid contains the coding sequence for signaling peptide M9, which contains the PVQE signaling motif.

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7CFE1_polio_VP12A

Plasmid

#212988

Purpose

Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts

Depositor

Karla Kirkegaard

Insert

Poliovirus type I Mahoney VP12A

Use

Mammalian cell free protein expression

Tags

HA tag

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-C17orf53_1-241-mCer

Plasmid

#211443

Purpose

mammalian expression of the N-terminal amino acids 1-241 of human C17orf53 C-terminally fused to mCerulean

Depositor

Helmut Pospiech

Insert

C17orf53 (HROB Human)

Tags

mCerulean

Expression

Mammalian

Mutation

Deletion of aa 242-646

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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