We narrowed to 15,993 results for: LEA

1. pTAT5

   Plasmid

   #112593

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-thioredoxin fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6xHis-thioredoxin

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. STK10 (STK10A-c043)

   Plasmid

   #110255

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   STK10 (STK10 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVP16-tev-PRT1-C195A

   Plasmid

   #83408

   Purpose

   bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

   Depositor

   Nico Dissmeyer

   Insert

   PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
   

   Tags

   His8, MBP, and tev cleavage site

   Expression

   Bacterial

   Mutation

   C195A substitution

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVP16-tev-PRT1-C29A

   Plasmid

   #83407

   Purpose

   bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

   Depositor

   Nico Dissmeyer

   Insert

   PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
   

   Tags

   His8, MBP, and tev cleavage site

   Expression

   Bacterial

   Mutation

   C29A substitution

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVP16-tev-PRT1-V28A

   Plasmid

   #83406

   Purpose

   bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies

   Depositor

   Nico Dissmeyer

   Insert

   PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
   

   Tags

   His8, MBP, and tev cleavage site

   Expression

   Bacterial

   Mutation

   V28A substitution

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. mKOet-LaminB1-10

   Plasmid

   #57928

   Purpose

   Localization: Nuclear Envelope, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mKOet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

7. PP-MAPK2 (3RP9)

   Plasmid

   #32862

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MAPK2
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   amino acids 123-548 only, P421L

   Available Since

   Jan. 10, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pBSG01

   Plasmid

   #37558

   Depositor

   Wei-Lih Lee

   Article

   Tang et al J Cell Biol. 2012 Mar 19;196(6):743-56.

   Insert

   PreScission protease cleavage site-S tag-TEV site-Z
   

   Expression

   Bacterial

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. TDRD7 (3RCO)

   Plasmid

   #32051

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD7 (TDRD7 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Available Since

   March 21, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pLIC.B3.CT0478

    Plasmid

    #11526

    Depositor

    Sung-Hou Kim

    Article

    Kim et al Proteins. 2006 Feb 1. 62(2):322-8.

    Insert

    ScpB
    

    Tags

    HisTag, TEV cleavable

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pSAB35

    Plasmid

    #175787

    Purpose

    Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

    Depositor

    John Rubinstein

    Article

    Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

    Insert

    SidK (1-278)
    

    Tags

    3xFLAG and 6xHis TEV

    Expression

    Bacterial

    Available Since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

    Plasmid

    #203652

    Purpose

    Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

    Depositor

    Liam Holt

    Article

    Shu T et et al bioRxiv 2022

    Insert

    PfV
    

    Use

    Lentiviral

    Tags

    Sapphire-IRES-H2B-mCherry

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4133611

    Plasmid

    #152146

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMAT11

    Plasmid

    #112592

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRMT10C (RG9MTD1A-c113)

    Plasmid

    #110248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RG9MTD1 (TRMT10C Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+ hnRNPK myc HIS

    Plasmid

    #71662

    Purpose

    Mammalian expression of hnRNPK

    Depositor

    Gideon Dreyfuss

    Insert

    hnRNPK (HNRNPK Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_crGE

    Plasmid

    #172933

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETA_crGE

    Plasmid

    #172927

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-Brainbow-2.1 R

    Plasmid

    #18723

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-Brainbow-1.1 M

    Plasmid

    #18722

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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