We narrowed to 19,051 results for: Tec

1. proISG15 (2-165)

   Plasmid

   #110762

   Purpose

   Bacterial expression of proISG15

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Tags

   N-His6-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

   Plasmid

   #163562

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-Venus-cpVenus-FLARE-ICUE

   Plasmid

   #123355

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-ICUE
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

   Plasmid

   #163554

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-NG-nCas9-RBMX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRIG-AktDN

   Plasmid

   #53597

   Purpose

   lentiviral expression of dominant negative mouse Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   Dominant negative (K179M)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pRS0045

   Plasmid

   #131124

   Purpose

   Mammalian Target-ACE expression vector

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Tags

   PmCDA1, TadA, and UGI

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

   Promoter

   CMV promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123329

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRIM28 gRNA (BRDN0001145369)

   Plasmid

   #76141

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pICE-HA-Ku70-siR-WT

    Plasmid

    #82329

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

    Insert

    Ku70 (XRCC6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Circular 200,100 (mPCSK9)

    Plasmid

    #170122

    Purpose

    AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA (Pcsk9 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human U6 and mouse U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. TBK1 gRNA (BRDN0001148607)

    Plasmid

    #76363

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PRKCE gRNA (BRDN0001148049)

    Plasmid

    #76791

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCE

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCE (PRKCE Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIRES-hrGFP II-mTET1_CD

    Plasmid

    #83571

    Purpose

    Expresses a mutant catalytic domain of TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    TET1 amino acids 1-1417 deleted, catalytic domain…

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTSK01K_0G7

    Plasmid

    #177296

    Purpose

    genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

    Depositor

    Karl-Erich Jaeger

    Article

    Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

    Insert

    PaacC1-aacC1
    

    Use

    Synthetic Biology; Yeast expression, tn7 genomic …

    Expression

    Bacterial

    Available Since

    Aug. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

    Plasmid

    #163561

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-HF-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307-SARS-CoV-2-3CL-C145A

    Plasmid

    #160279

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLAG-Foxo1-ADA 6KQ

    Plasmid

    #17563

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA 6KQ (Foxo1 Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available Since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only