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SFFV-AID-CTERM-GFP

Plasmid

#185768

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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XIAP (pBS0852)

Plasmid

#185282

Purpose

For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

XIAP (XIAP Human)

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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B*40:01:01

Plasmid

#203257

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*40:01:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 947 (pME-mmTcf4_DN_P2A_mCherry)

Plasmid

#232122

Purpose

A middle entry gateway clone containing myc-tagged murine dominant negative Tcf4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

Tcf4ΔN (Tcf4 Mouse)

Use

Gateway cloning

Tags

myc

Mutation

lacks aa 1-56

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3

Plasmid

#170175

Purpose

Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroME2f-ST-P2A-NLS-mRuby3

Use

AAV

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF45-2xCSTREP

Plasmid

#136206

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF45

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF45

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pGV3341

Plasmid

#170279

Purpose

Expression of mutant HIV-2 RT p66 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p66 (mutant)

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF10-2xCSTREP

Plasmid

#136174

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF10

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF10

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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MEIS1A-MIY

Plasmid

#21013

Depositor

Corey Largman

Article

Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

Insert

MEIS1A (Meis1 Mouse)

Use

Retroviral

Tags

IRES and YFP

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-Kaede

Plasmid

#219433

Purpose

AAV expression vector for Kaede

Depositor

Yoshiho Ikeuchi

Article

Martins-Costa et al Cell Stem Cell. 2024 Jun 6;31(6):866-885

Insert

Kaede

Use

AAV

Promoter

CAG

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Article

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Expression

Bacterial

Promoter

J23108 and PpsdA110

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2021

Plasmid

#49128

Purpose

AAV plasmid with DCX (Ple302) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple302-iCre

Use

AAV

Expression

Mammalian

Promoter

DCX

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGV3340

Plasmid

#170278

Purpose

Expression of mutant HIV-2 RT p51 reverse transcriptase

Depositor

Gregory Van Duyne

Article

Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

Insert

HIV-2 RT p51 (mutant)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7-lac

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCB’ CbbL Y72R

Plasmid

#162707

Purpose

Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

CbbL Y72R; Substitution of CsoS2-binding tyrosine…

Promoter

PLtet0-1 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB1_Guanylate-kin

Plasmid

#110036

Purpose

Protein expression and purification of CACNB1_Guanylate-kin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CACNB1_Guanylate-kin (CACNB1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K2-2xCSTREP

Plasmid

#136149

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K2

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K2

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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