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pMOD_A0408

Plasmid

#91010

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0708

Plasmid

#91021

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-srlA

Plasmid

#89965

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

srlA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rbsK

Plasmid

#89963

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rbsK gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-yhhT

Plasmid

#89958

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

yhhT gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-torZ

Plasmid

#89956

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

torZ gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-arsB

Plasmid

#89952

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

arsB gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-WNT1

Plasmid

#79499

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

WNT1 (WNT1 Human)

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCas59

Plasmid

#82396

Purpose

sgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCas57

Plasmid

#82395

Purpose

sgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas56

Plasmid

#82394

Purpose

sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas55

Plasmid

#82393

Purpose

sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCas52

Plasmid

#82392

Purpose

sgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

gRNA

Promoter

PEZ3

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBW301ParsR-Amp30T

Plasmid

#78653

Purpose

pSB3K3 carrying araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

araC-PBAD-30hrpV-t-J101-32arsR-t-ParsR-

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 1, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (Y1142fs)

Plasmid

#73969

Purpose

STAG2 cDNA (CCDS43990) Y1142fs

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

Y1142fs

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (I885fs)

Plasmid

#73966

Purpose

STAG2 cDNA (CCDS43990) I885fs

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

I885fs

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S202L)

Plasmid

#73972

Purpose

STAG2 cDNA (CCDS43990) S202L

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S202L

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (V181M)

Plasmid

#73971

Purpose

STAG2 cDNA (CCDS43990) V181M

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Tags

FLAG-SBP

Expression

Mammalian

Mutation

V181M

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only