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We narrowed to 18,123 results for: multi

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Showing: 9141 - 9160 of 18123 results

  1. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid
    #182395
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    Tags
    Vesicle Nucleating peptide (VNp) & Leucine Zi…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182395-plasmid-map-sequence-id-364922

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  3. LeGO-iBSD-5LO-WT

    Plasmid
    #182741
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (WT) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182741-plasmid-map-sequence-id-360423

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  4. pEF1α-M2rtTA-T2A-Puro

    Plasmid
    #169175
    Purpose
    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    M2rtTA-T2A-Puro
    Use
    Piggybac transposon
    Expression
    Mammalian
    Mutation
    See Depositor Comments Below
    Promoter
    EF1a
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169175-plasmid-map-sequence-id-362474

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  7. pNOC_dCas9_sgRNA NC

    Plasmid
    #176259
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176259-plasmid-map-sequence-id-346581

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  8. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172316-plasmid-map-sequence-id-343650

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  12. VRQR-HF1 variant

    Plasmid
    #138563
    Purpose
    Expresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    VRQR-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138563-plasmid-map-sequence-id-274789

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  16. pHH29_32 series plasmid

    Plasmid
    #120901
    Purpose
    Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device
    Depositor
    Domitilla Del Vecchio
    Article
    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:
    Inserts
    Ec-TTL-P109
    sRNA-A targeting sequence
    RBS TIR=974 of GFPop1 gene
    GFPop1
    RBS TIR=6474 of ECF32mut gene
    ECF32mut
    T7 terminator
    resource competitor
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    120901-plasmid-map-sequence-id-241429

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  18. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Available Since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  20. pSV40-GUS-P2A-Hygro

    Plasmid
    #106480
    Purpose
    Expression of GUS and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    beta-glucuronidase
    Hygromycin B resistance
    Expression
    Mammalian and Plant
    Promoter
    SV40 promoter
    Available Since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106480-plasmid-map-sequence-id-207984

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Showing: 9141 - 9160 of 18123 results