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WEE1 gRNA (BRDN0001149349)

Plasmid

#77367

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WEE1 (WEE1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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p260 pCMV-CRE-M-AR(wt)

Plasmid

#12494

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

CRE-M AR (AR Human)

Use

Cre/Lox

Expression

Mammalian

Mutation

CRE-M and AR ligand binding domain

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
WT N-His Ana GvpC in pET28a

Plasmid

#153294

Purpose

Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (N-terminal his-tagged)

Tags

His-tag

Expression

Bacterial

Mutation

Added a hexahistidine tag after the methionine at…

Promoter

T7

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001146465)

Plasmid

#77174

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001146597)

Plasmid

#77175

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PIM1 gRNA (BRDN0001145078)

Plasmid

#77176

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIM1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIM1 (PIM1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pF714-pET28a-Hs-FL-METTL20-NHis wt

Plasmid

#85115

Purpose

expression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length METTL20 (METTL20 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only
pF711-pET28a-Hs-delta25-MCAD-NHis wt

Plasmid

#85112

Purpose

expression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta25) MCAD (ACADM Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE Tight ArchT-GFP

Plasmid

#79627

Purpose

Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

Depositor

Yasunori Hayashi

Article

Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

Insert

ArchT

Tags

EGFP

Expression

Mammalian

Mutation

ArchT is a more sensitive version of Arch (archae…

Promoter

Tet responsive Ptight promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only
non-targeting control gRNA (BRDN0001145869)

Plasmid

#80256

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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ROCK1 gRNA (BRDN0001147709)

Plasmid

#77593

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK1 (ROCK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

Plasmid

#205463

Purpose

A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-A; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

Plasmid

#205465

Purpose

A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

Depositor

Bin Shen

Article

Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

Insert

MTS; N136; ccdb; RsDddA-C; UGI

Expression

Mammalian

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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WEE1 gRNA (BRDN0001145570)

Plasmid

#77368

Purpose

3rd generation lentiviral gRNA plasmid targeting human WEE1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WEE1 (WEE1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A14

Plasmid

#132105

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

Insert

SLC39A14 (SLC39A14 Human)

Expression

Mammalian

Available Since

Dec. 3, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

Plasmid

#187994

Purpose

Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

mucin2 D1D2D'D3 (MUC2 Human)

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK7a1-EE

Plasmid

#47578

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK7a1-EE (Map2k7 Mouse)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Residues 73-420 of RefSeq sequence. Ser271 to Gl…

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
PLK1 gRNA (BRDN0001145759)

Plasmid

#76457

Purpose

3rd generation lentiviral gRNA plasmid targeting human PLK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PLK1 (PLK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLD1_1

Plasmid

#160791

Purpose

Suppress POLD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLD1_1

Use

Lentiviral

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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