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MSCV-PIG-METTL14-WT

Plasmid

#141118

Purpose

Retroviral overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

METTL14 (METTL14 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 scramble (plus strand)

Plasmid

#91852

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0347)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 scramble (plus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Sequence scrambled from ch10:6094588-6094934

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-IL2RA CaRE4 (minus strand)

Plasmid

#91849

Purpose

Luciferase reporter for IL2RA enhancer (IGI-P0626)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

IL2RA CaRE4 (minus strand) (IL2RA Human)

Use

Luciferase

Expression

Mammalian

Mutation

Perfect match to reference sequence

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23-CD69 CaRE3 (plus strand)

Plasmid

#91844

Purpose

Luciferase reporter for CD69 enhancer (IGI-P0621)

Depositor

Jacob Corn

Article

Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

Insert

CD69 CaRE3 (plus strand) (CD69 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Missing 1 base from 13 base polyT tract beginning…

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRAU171

Plasmid

#86835

Purpose

bacterial expression of anti-CRISPR protein AcrIIA2

Depositor

Joseph Bondy-Denomy

Article

Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

Insert

acrIIA2

Expression

Bacterial

Promoter

araBAD

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728)

Plasmid

#248232

Purpose

Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

None

Promoter

NA

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

Plasmid

#248211

Purpose

bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX307-RAD54L2-FL-SFB

Plasmid

#247912

Purpose

Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

RAD54L2 (RAD54L2 Human)

Use

Lentiviral

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Vcan-V3-HA

Plasmid

#238076

Purpose

Expresses Vcan V3 in mice heart tissues

Depositor

Yuxuan Guo

Article

Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

Insert

Vcan (Vcan Mouse)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-SB

Plasmid

#220125

Purpose

Expresses the 1.3-kDa Small BiT (SB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-SB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-Gx-LB

Plasmid

#220126

Purpose

Expresses the 18-kDa Large BiT (LB) fragment of split NanoLuc, genetically fused via a semi-rigid peptide linker to a protein G adapter domain (Gx).

Depositor

Maarten Merkx

Article

Ni et al Nat Commun. 2021 Jul 28;12(1):4586. doi:

Insert

Gx-LB

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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hyA&C-BEmax

Plasmid

#196097

Purpose

Expresses hyA&C-Bemax

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

hyA&C-BEmax

Use

CRISPR

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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eA&C-BEmax

Plasmid

#196098

Purpose

Expresses eA&C-BEmax

Depositor

Xiahui Zhang

Article

Xue et al Nat Commun. 2023 Mar 3;14(1):1224. doi:

Insert

eA&C-BEmax

Use

CRISPR

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRSET A-pcStar

Plasmid

#226339

Purpose

Bacterial expression of RSFP pcStar; photoconvertible red fluorescent protein with high brightness, high photoconversion contrast, and early detectable fluorescence

Depositor

Pingyong Xu

Article

Zhang et al Nano Lett. 2020 Apr 8;20(4):2197-2208. d

Insert

pcStar

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-YTHDF2

Plasmid

#238975

Purpose

Expresses YTHDF2

Depositor

Jianjun Chen

Article

Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

Insert

YTHDF2 (YTHDF2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.H66A.L276V

Plasmid

#238944

Purpose

Green glucose sensor, high sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(NLS)iGlucoSnFR.L276V.K312A

Plasmid

#238945

Purpose

Green glucose sensor, moderate sensitivity, nuclear

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc and NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Bcl6

Plasmid

#137851

Purpose

Express Bcl6 in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

B cell lymphoma 6 (BCL6 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Prkd2 without kinase domain

Plasmid

#138411

Purpose

Express Prkd2 without kinase domain in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Prkd2 without kinase domain (PRKD2 Human)

Tags

HA

Expression

Mammalian

Mutation

kinase domain deleted from human Prkd2

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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