We narrowed to 9,363 results for: mel

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TFORF0677

Plasmid

#142192

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRDM16 (PRDM16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDNR-SpCas9-Cdt1

Plasmid

#80426

Purpose

Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Insert

SpCas9-Cdt1

Use

CRISPR

Tags

Cdt1

Mutation

codon optimized for human

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mH33B-mClover3

Plasmid

#171496

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

H3.3B (H3f3b Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0676

Plasmid

#142191

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PRDM16 (PRDM16 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mClover3-MAP4-MTBD

Plasmid

#171498

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Article

So et al Science. 2019 Jun 28;364(6447). pii: 364

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mClover3

Promoter

T7

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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XIAP (pBS0852)

Plasmid

#185282

Purpose

For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

XIAP (XIAP Human)

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218864

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pAcUW51-gEgIL

Plasmid

#11622

Depositor

Pamela Bjorkman

Article

Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

Insert

HSV-1 glycoprotein E and HSV-1 glycoprotein I

Tags

His

Expression

Insect

Mutation

Dicistronic vector containing HSV-1 gE (KOS strai…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE731

Plasmid

#103008

Purpose

S. cerevisiae Episomal plasmid harboring Fncpf1 under the control of TDH3p

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

Fncpf1

Tags

3HA and NLS

Expression

Yeast

Mutation

human codon optimized

Promoter

TEF1

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218865

Purpose

LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Promoter

-

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_47-300 (pBS0847)

Plasmid

#185279

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_47-300

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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HBM-mVEGF-C-HisTag-pENTR

Plasmid

#64066

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

Plasmid

#185328

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA_81-236-FLAG

Tags

FLAG

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[G249D]-P2A-Electra1 (pAH10)

Plasmid

#227615

Purpose

Overexpression of SLC25A46 with G249D mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

G249D

Promoter

CMV, T7

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

Plasmid

#227617

Purpose

Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

R340C

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

Plasmid

#227618

Purpose

Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

I349D

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

Plasmid

#227619

Purpose

Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

deleted aa 2-83

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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