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pGGDestSC-ATG

Plasmid

#49322

Purpose

Destination vector for Golden Gate assembly; Few restriction sites for subcloning.

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

Type

Empty backbone

Use

Destination vector

Tags

Expression

Mutation

Promoter

Available since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-RQ/TG

Plasmid

#29397

Depositor

Mark Cookson

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Thr1343Gly Arg1398Gln

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K533A

Plasmid

#14100

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2Q545A

Plasmid

#14101

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Gln545 to Ala

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K533AQ545A

Plasmid

#14103

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Gln545 to Alanine

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pEF.DEST51-YPet

Plasmid

#215541

Purpose

Expression vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-mTurquoise2

Plasmid

#215539

Purpose

Expression vector with the mTurquoise2 fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11

Plasmid

#215523

Purpose

Expression vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Dok1-V2

Plasmid

#215452

Purpose

Expression vector with Dok1 tagged with the BiFC V2 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Use

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-Dok1

Plasmid

#215458

Purpose

Expression vector with Dok1

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pPB.DEST-Illusia

Plasmid

#215444

Purpose

Piggybac expression vector with Illusia FRET reporter for integrin phosphorylation

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Illusia

Use

Transposon-based stable expression

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SRGAP2C_pEF-DEST51

Plasmid

#238497

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

SRGAP2C (SRGAP2C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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ARHGAP11B_pEF-DEST51

Plasmid

#238498

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

ARHGAP11B (ARHGAP11B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-TDP-43-HA

Plasmid

#235496

Purpose

Expression of human TDP-43-HA fusion protein

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDest17_N-myc

Plasmid

#234045

Purpose

Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

Depositor

Tanja Mittag

Article

Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

Insert

N-myc (MYCN Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 30mer

Plasmid

#231888

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 30mer

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 StoL

Plasmid

#231882

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 StoL

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 StoL

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104L, S117L, S136L, S144L

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 S/ArotoL

Plasmid

#231880

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 S/ArotoL

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap1

Plasmid

#231879

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Swap1

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol W

Plasmid

#231878

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 solvvol W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 solvvol

Plasmid

#231877

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 solvvol

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L113A+

Plasmid

#231873

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L113A+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 L113A+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 E2K2

Plasmid

#231868

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 E2K2

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 E2K2

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S104K, S117E, S136E, S144K

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 9acidA

Plasmid

#231867

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 9acidA

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 9acidA

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 W120A

Plasmid

#231862

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 W120A

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

W120A

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Aro3+

Plasmid

#231884

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Aro3+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Aro2+

Plasmid

#231883

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Aro2+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Aro2+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 WTSLFD

Plasmid

#231881

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 WTSLFD

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108W, E109T, Y110S, E111L, N112F, L113D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 F108A+

Plasmid

#231875

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F108A+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 F108A+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F108A, T121L, A141P

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 ILVtoED W

Plasmid

#231871

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoED W

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

N126W, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 L123A+

Plasmid

#231869

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L123A+

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 L123A+

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123A

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Swap2

Plasmid

#231865

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap2

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Swap2

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 F124I

Plasmid

#231864

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F124I

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F124I

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 Swap3

Plasmid

#231860

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap3

Depositor

Tanja Mittag

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 Swap3

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 ILVtoAS

Plasmid

#231859

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoAS

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 ILVtoED

Plasmid

#231857

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 ILVtoED

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 WT 44mer

Plasmid

#231856

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 44mer

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4 44mer

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_FL Gcn4

Plasmid

#231855

Purpose

Bacterial expression of N-terminally 6His tagged FL Gcn4

Depositor

Tanja Mittag, Aseem Ansari

Article

Bremer et al bioRxiv 2024.11.21.624739

Insert

Gcn4

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-pDEST_BirA_Flag

Plasmid

#227960

Purpose

BioID experiment

Depositor

Alessandro Ori

Insert

BirA

Use

Tags

Expression

Mammalian

Mutation

R118G

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-pDEST_BirA_Flag_SLC45A1

Plasmid

#227959

Purpose

BioID experiment

Depositor

Alessandro Ori

Insert

SLC45A1 (SLC45A1 Human)

Use

Tags

BirA-FLAG

Expression

Mammalian

Mutation

R118G in BirA

Promoter

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1801 - 1840 of 274993 results

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